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麦冬须根脂溶性成分研究

段昌令1,2, 姜宇3, 姜勇1, 林成仁2, 刘建勋2, 屠鹏飞1*   

  1. 1. 北京大学 药学院, 北京 100191
    2. 中国中医科学院 西苑医院, 北京 100091
    3. 沈阳药科大学 中药学院, 辽宁 沈阳 110015
  • 收稿日期:2009-05-04 修回日期:2009-08-10 出版日期:2009-09-15 发布日期:2009-09-15
  • 通讯作者: 屠鹏飞*

Liposoluble chemical constituents from the fibrous root of Ophiopogon japonicas

Chang-Ling Duan1,2, Yu Jiang3, Yong Jiang1, Cheng-Ren Lin2, Jian-Xun Liu2, Peng-Fei Tu1*   

  1. 1. School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
    2. Xiyuan Hospital, Academy of Chinese Medical Sciences, Beijing 100091, China
    3. School of Traditional Chinese Materia Medicas, Shenyang Pharmaceutical University, Shenyang 110015, China
  • Received:2009-05-04 Revised:2009-08-10 Online:2009-09-15 Published:2009-09-15
  • Contact: Peng-Fei Tu*

摘要:

我们对麦冬(Ophiopogon japonicus) 须根的脂溶性成分进行研究。 应用硅胶、Sephadex LH-20、ODS柱以及半制备液相色谱进行分离和纯化, 应用波谱技术确定化合物的结构。从中分离鉴定了10个化合物, 分别是: 2'-羟基麦冬黄酮 A (1), 5,8-dimethoxy-6-methyl-7-hydroxy-3-(2'-hydroxy-4'-methoxybenzyl) chroman-4-one (2), 5,7-dihydroxy-6,8-dimethyl-3-(4'-hydroxybenzyl) chroman-4-one (3), 7,4'-dihydroxy-5-methoxyflavanone (4), N-trans-coumaroyltyramine (5), N-trans-coumaroyloctopamine (6), N-trans-feruloyltyramine (7), 对羟基桂皮酸 (8), 咖啡酸(9), 阿魏酸 (10)。化合物4, 6, 7, 9, 10 首次从沿阶草属中分离得到。利用Hella、Hep2肿瘤细胞测定化合物1, 2, 47, 9, 10 的细胞毒作用, 1, 2, 57, 9 显示一定的细胞毒活性。

关键词: 百合科, 沿阶草属, 须根, 化学成分, 细胞毒活性

Abstract:

We investigated the chemical constituents of the fibrous root of Ophiopogon japonicus and their cytotoxicities against Hela and Hep2 cells. Ten compounds were purified by various chromatographic techniques. Their structures were identified as 2'-hydroxylophiopogonone A (1), 5,8-dimethoxy-6-methyl-7-hydroxy-3-(2'-hydroxy-4'-methoxybenzyl) chroman-4-one (2), 5,7-dihydroxy-6,8-dimethyl-3-(4'-hydroxybenzyl) chroman-4-one (3), 7,4'-dihydroxy-5-methoxyflavanone (4), N-trans-coumaroyltyramine (5), N-trans-coumaroyloctopamine (6), N-trans-feruloyltyramine (7), 4-hydroxycinnamic acid (8), caffeic acid (9), and ferulic acid (10) on the basis of spectroscopic analyses. Compounds 4, 6, 7, 9, 10 were obtained from this genus for the first time. The cytotoxic activities of 1, 2, 4, 5, 6, 7, 9 and 10 against Hela and Hep2 cells are described.

Key words: Liliaceae, Liliaceae, Ophiopogon japonicus, Ophiopogon japonicus, Fibrous root, Fibrous root, Chemical constituents, Chemical constituents, Cytotoxicity, Cytotoxicity

中图分类号: 

Supporting:

Foundation item: Program for Changjiang Scholar and Innovative Team in University (Grant No. 985-2-063-112).
*Corresponding author. Tel.: 86-10-82805189; e-mail: pengfeitu@bjmu.edu.cn