中药三七化学成分的HPLC/ESI-MS分析

摘要/Abstract

摘要： 目的建立分析中药三七的HPLC-UV-MS方法, 鉴定三七的化学成分。方法色谱柱: Phenomenex Luna C18 柱 (250 mm×4.6 mm ID, 5 μm); 流动相: 0.005%甲酸水溶液(A) 和0.005%甲酸乙腈溶液(B), 梯度洗脱; 紫外检测波长范围:195-400 nm; 采用正负离子检测模式。结果三七成分获得了良好的分离与检测。通过与对照品的保留时间、正负离子质谱比较, 鉴定了14个成分, 根据正负离子质谱数据和文献, 分析推断了41个成分, 其中, 野三七皂苷-H、野三七皂苷-E、竹节参皂苷-L5、丙二酰基人参皂苷-Rg1、以及三七皂苷-J、-A、-R1、-G、-R2和人参皂苷-Rh3的异构体为首次在三七中发现。结论本方法灵敏度高、分离度好, 适合三七成分的鉴定。本结果有助于进一步对三七进行活性成分研究及质量控制。

关键词: 三七, 三七, 三七, 液质联用, 液质联用, 液质联用, 人参皂苷, 人参皂苷, 人参皂苷, 三七皂苷, 三七皂苷, 三七皂苷, 黄酮, 黄酮, 黄酮

Abstract: Aim To develop a HPLC-UV-MS method for identifying the constituents in the Chinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C18 column (250 mm×4.6 mm ID, 5 μm) was utilized. Water containing 0.005% formic acid (A) and acetonitrile containing 0.005% formic acid (B) were used as gradient eluents. UV spectra were recorded in range 195-400 nm. Both positive and negative ion ESI modes were used. Results The constituents in Notoginseng were well separated and detected. Fourteen compounds were identified by comparing their retention time and ESI-MS data with those obtained from the reference compounds. Forty-one compounds were deduced by data analysis of MS and literature; among them, yesanchinosides-H and -E, chikusetsusaponin-L5, malonyl-ginsenoside-Rg1, the isomers of notoginsenosides-J, -A, -R1, -G, -R2, and ginsenoside-Rh3 were discovered in Notoginseng for the first time. Conclusion This method gives high sensitivity and good separation, and is suitable for identifying the constituents in Notoginseng. This result is helpful for further phytochemical research on Notoginseng. Based on this result, further quality control can be studied.

Key words: Panax notoginseng, Panax notoginseng, HPLC-MS, HPLC-MS, ginsenosides, ginsenosides, notoginsenosides, notoginsenosides, flavonoids, flavonoids

中图分类号:

R284.1

R917.01

Supporting: Foundation item: National Basic Research Program of China ( No.G1999054406) National Natural Science Foundation of China (No.39970898).
^*Corresponding author. Tel.: 86-10-82801711

刘进怀, 王璇^*, 蔡少青, 小松かつ子, 难波恒雄. 中药三七化学成分的HPLC/ESI-MS分析[J]. .

LIU Jin-huai, WANG Xuan^*, CAI Shao-qing, KOMATSU Katsuko, NAMBA Tsuneo. Analysis of the Constituents in the Chinese Drug Notoginseng by Liquid Chromatography-Electrospray Mass Spectrometry[J]. .

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