艾纳香二氢黄酮对脂质过氧化损伤的恒河猴原代培养肝细胞及肝亚细胞的保护作用

艾纳香二氢黄酮对脂质过氧化损伤的恒河猴原代培养肝细胞及肝亚细胞的保护作用

赵金华, 许实波^*, 王正濂, 林永成, 陈荣礼

1. 中山大学药学系, 广州 510275;
2. 香港理工大学应用生物化学技术系, 香港

收稿日期:1997-04-14 修回日期:1998-01-20 出版日期:1998-09-15 发布日期:1998-09-15
通讯作者: 许实波*

Protective Actions of Blumea Flavanones on Primary Cultured Hepatocytes and Liver Subcellular Organelle against Lipid Peroxidation

Jin-Hua Zhao, Shi-Bo Xu^*, Zheng-Lian Wang, Yong-Cheng Lin, Rong-Li Chen

1. Department of Pharmacy; Zhongshan University; Guangzhou 510275;
2. Department of Applied Biology and Chemical Technology; Hong Kong

Received:1997-04-14 Revised:1998-01-20 Online:1998-09-15 Published:1998-09-15
Contact: Shi Bo Xu*

摘要/Abstract

摘要： 目的: 研究艾纳香二氢黄酮对脂质过氧化致损伤的恒河猴原代培养肝细胞及肝亚细胞的保护作用。方法: 艾纳香二氢黄酮与原代培养肝细胞共育后, 以CCl4 或FeSO4-半胱氨酸致细胞损伤, 以脂质过氧化产物丙二醛产生量及转氨酶的漏出量作为损伤程度指标。亚细胞结构与艾纳香二氢黄酮共育后, 以FeSO4-半胱氨酸致脂质过氧化, 以丙二醛产生量衡量过氧化损伤程度。结果: 10 及100 μmol·L^-1的艾纳香二氢黄酮可降低被损伤的肝细胞中的丙二醛产生, 降低转氨酶漏出, 抑制肝亚细胞结构中丙二醛生成。结论: 艾纳香二氢黄酮具有抑制脂质过氧化作用及肝细胞、亚细胞结构保护作用。

关键词: 艾纳香二氢黄酮, 抗过氧化剂, 恒河猴, 肝细胞, 亚细胞

Abstract: To search for the protective actions of blumea flavanones (BFs) on hepatocytes and hepatic subcellular organelle against lipid peroxidation, monkey's hepatocytes were isolated and cultured with or without blumea flavanones, then damaged by FeSO4-cysteine or CCl4. The lipid peroxidation (malondialdehyde production) and alteration in hepatocyte membrane (leakage of GPT) were estimated. Hepatic subcellular organelles were also isolated and incubated with or without blumea flavanones, then injured by FeSO4-ascorbate. The generation of malondialdehyde(MDA) was measured. It was found that BFs 10 and 100 μmol·L^-1 inhibited the MDA generation and GPT (glutamic pyruvic transaminase) leakage out of hepatocytes that were induced by CCl4 or FeSO4-cysteine. BFs could prevent lipid peroxidation initiated by FeSO4-ascorbate in subcellular organelle suspension. Among BFs, BF-possessed the strongest activity. Conclusion: Blumea flavanones possess antioxidation activities that protect monkey's hepatocytes and hepatic subcellular organelle against injuries induced by FeSO4 or CCl4.

Key words: Blumea flavanones, Antioxidants, Macaca mulatta, Hepatocyte, Subcellular organelle

Supporting:

赵金华, 许实波^*, 王正濂, 林永成, 陈荣礼. 艾纳香二氢黄酮对脂质过氧化损伤的恒河猴原代培养肝细胞及肝亚细胞的保护作用[J]. .

Jin-Hua Zhao, Shi-Bo Xu^*, Zheng-Lian Wang, Yong-Cheng Lin, Rong-Li Chen . Protective Actions of Blumea Flavanones on Primary Cultured Hepatocytes and Liver Subcellular Organelle against Lipid Peroxidation[J]. .

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