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爱大霉素和庆大霉素对肾皮质内质网45Ca2+摄取及Ca2+-Mg2+-ATPase活性的影响

李忠东, 洪文清, 李培忠   

  1. 1. 空军总医院药理科, 北京 100036;
    2. 军事医学科学院毒物药物研究所, 北京 100850
  • 收稿日期:1997-04-07 修回日期:1998-03-03 出版日期:1998-09-15 发布日期:1998-09-15

The Effects of Etimicin and Gentamicin on Renal Endoplasmic Reticulum 45Ca2+-Uptake and Ca2+-Mg2+-ATPase Activity

Zhong-Dong Li, Wen-Qing Hong, Pei-Zhong Li   

  1. 1. Department of Clinical Pharmacology, General Hospital of Air Force, Beijing 100036;
    2. Institute of Toxicology and Pharmacology, Academy of Military Medical Science, Beijing100850
  • Received:1997-04-07 Revised:1998-03-03 Online:1998-09-15 Published:1998-09-15

摘要: 目的: 研究爱大霉素和庆大霉素对大鼠肾皮质内质网45Ca2+摄取以及对内质网膜上Ca2+-Mg2+-ATPase活性的影响。方法: 45Ca2+示踪技术和孔雀蓝分光光度法。结果: 爱大霉素和庆大霉素在大于或等于3.4×10-4 mol·L-1, 能抑制内质网45Ca2+摄取(抑制率分别大于或等于17.4%25.5%); 3.4×10-2 mol·L-1, 对内质网膜上Ca2+-Mg2+-ATPase活性有抑制作用(抑制率分别为24.17%29.19%)结论: 爱大霉素和庆大霉素在较高浓度时可使胞浆钙升高, 这可能与其产生肾毒性有关。

关键词: 爱大霉素, 庆大霉素, 肾皮质内质网, 45Ca2+摄取, Ca2+-Mg2+-ATPase

Abstract: Objective: To study the effects of etimicin (EM) and gentamicin (GM) on renal endoplasmic reticulum 45Ca2+ uptake and Ca2+-Mg2+-ATPase activity. Methods: Using 45Ca2+ incorporation technique and peacock blue spectrophotometry respectively. Results: EM and GM (≥3.4×10-4 mol·L-1) inhibited endoplasmic reticulum 45Ca2+-uptake (the rate of inhibition: ≥17.4% and ≥25.5%, respectively); EM and GM (3.4×10-2 mol·L-1) inhibited Ca2+-Mg2+-ATPase activity (the rate of inhibition: 24.2% and 29.2%, respectively). Conclusion: At high concentration, EM and GM elevated intracellular calcium which may be related to their nephrotoxicity.

Key words: Etimicin (EM), Gentamicin (GM), Endoplasmic reticulum, 45Ca2+-uptake, Ca2+-Mg2+-ATPase

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