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多巴胺D2受体三维结构预测及与激动剂配体相互作用的研究

朱七庆, 郭宗儒*   

  1. 中国医学科学院 北京协和医科大学药物研究所, 北京 100050
  • 收稿日期:1997-06-17 修回日期:1998-04-10 出版日期:1998-09-15 发布日期:1998-09-15
  • 通讯作者: 郭宗儒*

Modeling of Dopamine D2 Receptor and its Agonist DOCK Analyses

Qi-Qing Zhu, Zong-Ru Guo*   

  1. Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050
  • Received:1997-06-17 Revised:1998-04-10 Online:1998-09-15 Published:1998-09-15
  • Contact: Zong-Ru Guo*

摘要: 以细菌视紫红质(bacteriorhodopsin)的三维晶体结构为模板, 预测了多巴胺D2受体跨膜区的七个α螺旋肽段的三维结构。根据定点突变实验数据以及预测的受体三维结构, 确定了激动剂配体结合腔由Asp86, Ser141, Ser144等十二个氨基酸残基组成。为了校正和检验所得的模型, 分别以一组刚性、一组柔性的激动剂与受体对接(DOCK), 分析-logIC50和结合能Eb相关性, 较好的结果说明该模型是可靠的。

关键词: 多巴胺D2受体, 三维结构预测, 对接

Abstract: A model of transmembrane helices of dopamine D2 receptor was constructed using the X-ray coordinates of bacteriorhodopsin (BR) as a template. Based on the results from the model and the sitedirected mutagenesis experience, the binding pocket, including nine amino acid residues beside indispensable Asp86, Ser141 and Ser144 residues, was defined. In order to testify the 3D-structure of dopamine D2 receptor and specially test the binding sites, two sets of D2 receptor agonists (one was rigid and the other flexible) were selected for docking. A good result of correlation between -logIC50 and binding energy Eb indicates that the predicted model is reliable for the investigation of the receptor-ligand interaction and design of new active molecules.

Key words: Dopamine D2 receptor, 3D-structure prediction, DOCK

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