地黄饮子对db/db小鼠海马和视网膜神经元凋亡机制的研究

doi:10.5246/jcps.2024.10.066

中国药学(英文版) ›› 2024, Vol. 33 ›› Issue (10): 918-931.DOI: 10.5246/jcps.2024.10.066

地黄饮子对db/db小鼠海马和视网膜神经元凋亡机制的研究

王小丹¹^,², 李钦青¹^,², 桂婉威¹^,², 武东燕¹^,², 柴金苗¹^,²^,^*(), 贺文彬¹^,², 张俊龙¹^,²^,^*()

^1. 山西中医药大学, 分子中医药学国家国际联合研究中心, 山西太原 030024
^2. 山西中医药大学, 中医脑病学山西省重点实验室, 山西太原 030024

收稿日期:2024-03-12 修回日期:2024-04-15 接受日期:2024-06-23 出版日期:2024-10-31 发布日期:2024-10-31
通讯作者: 柴金苗, 张俊龙

Investigation of the apoptotic mechanism in hippocampal and retinal neurons of db/db mice induced by Dihuangyinzi decoction

Xiaodan Wang¹^,², Qinqing Li¹^,², Wanwei Gui¹^,², Dongyan Wu¹^,², Jinmiao Chai¹^,²^,^*(), Wenbin He¹^,², Junlong Zhang¹^,²^,^*()

¹ National International Joint Research Center of Molecular Chinese Medicine, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, Shanxi, China
² Shanxi Key Laboratory of Chinese Brain Diseases, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, Shanxi, China

Received:2024-03-12 Revised:2024-04-15 Accepted:2024-06-23 Online:2024-10-31 Published:2024-10-31
Contact: Jinmiao Chai, Junlong Zhang
Supported by:
National Natural Science Foundation (Grant No. 8227153727; 81874420).

摘要/Abstract

摘要：

中文摘要

关键词: db/db小鼠, 地黄饮子, 凋亡, 视网膜, 海马

Abstract:

abstract

Key words: db/db mice, Dihuangyinzi, Apoptosis, Retina, Hippocampus

Supporting:

王小丹, 李钦青, 桂婉威, 武东燕, 柴金苗, 贺文彬, 张俊龙. 地黄饮子对db/db小鼠海马和视网膜神经元凋亡机制的研究[J]. 中国药学(英文版), 2024, 33(10): 918-931.

Xiaodan Wang, Qinqing Li, Wanwei Gui, Dongyan Wu, Jinmiao Chai, Wenbin He, Junlong Zhang. Investigation of the apoptotic mechanism in hippocampal and retinal neurons of db/db mice induced by Dihuangyinzi decoction[J]. Journal of Chinese Pharmaceutical Sciences, 2024, 33(10): 918-931.

图/表 19

Figure 1. Plot of six different trial types and correct position objects in S-PAL and D-PAL. Note: S+ is indicated by A.

Table 1. Primer sequences for RT-qPCR.

Table 2. Results of S-PAL in db/db mice (x ± s, n = 5).

Table 3. Results of D-PAL in db/db mice (x ± s, n = 5).

Table 4. Response times and delay times between S-APL and D-APL in db/db mice (x ± s, n = 5).

Table 5. Changes in fasting blood glucose of the mice in each group (x ± s, n = 6).

Figure 2. Effect of DHYZ on CA1 gion of db/db mouse hippocampus (H&E staining, 400). Note: "a" is the neuronal cells.

Figure 3. Effect of DHYZ on db/db mice retina (H&E staining, 400). Note: The green arrow indicates neo-angiogenesis.

Figure 4. Effect of DHYZ on hippocampal neurons in the CA1 region of db/db mice (Nissl staining, 400).

Figure 5. Effect of DHYZ on retinal neurons of db/db mice (Nissl staining, 400). Note: The red arrow is the Nissl body.

Figure 6. Bcl-2 positive expression in the CA1 region of the hippocampus of db/db mice (immunohistochemistry, 400).

Figure 7. Bax positive expression in CA1 region of the hippocampus of db/db mice (immunohistochemistry, 400).

Figure 8. Positive Bcl-2 expression in db/db mouse retina (Immunohistochemistry, 400).

Figure 9. Positive Bax expression in db/db mouse retina (immunohistochemistry, 400).

Table 6. Positive expression levels of Bcl-2 and Bax in each group (x ± s, n = 6).

Table 7. Changes in fasting blood glucose of the mice in each group (x ± s, n = 6).

Figure 10. Expression levels of proteins associated with the JNK/p38 MAPK signaling pathway in db/db mice hippocampus (x ± s, n = 6).

Figure 11. Expression levels of proteins associated with the JNK/p38 MAPK signaling pathway in db/db mice retina (x ± s, n = 6).

Table 8. Comparison of JNK/p38 MAPK pathway-related protein levels in the hippocampus and retinal tissues of mice (x ± s, n = 6).

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地黄饮子对db/db小鼠海马和视网膜神经元凋亡机制的研究

Investigation of the apoptotic mechanism in hippocampal and retinal neurons of db/db mice induced by Dihuangyinzi decoction

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