猪胎盘肽通过提高TrkB和BDNF水平及促进神经母细胞增殖和分化延缓D-半乳糖诱导的小鼠衰老

doi:10.5246/jcps.2019.10.070

中国药学(英文版) ›› 2019, Vol. 28 ›› Issue (10): 739-748.DOI: 10.5246/jcps.2019.10.070

• 【研究论文】 • 上一篇

猪胎盘肽通过提高TrkB和BDNF水平及促进神经母细胞增殖和分化延缓D-半乳糖诱导的小鼠衰老

权栩¹, 顾彩虹³, 严峰⁴, 高垚垚⁵, 陈琳⁶, 卫榕⁷, 颜丙春^1,2*, 胡荣^1,2*

1. 扬州大学医学院转化医学研究院药学系, 江苏扬州 225001
2. 国家中医药管理局胃癌毒邪论治重点研究室, 江苏扬州 225001
3. 江苏连环药业集团有限公司, 江苏扬州 225009
4. 江苏扬农化工集团有限公司, 江苏扬州 225001
5. 高邮市中医医院, 江苏扬州 225600
6. 江苏省南通市通州区人民医院, 江苏南通 226300
7. 南通市肿瘤医院, 江苏南通 226361

收稿日期:2019-06-30 修回日期:2019-08-27 出版日期:2019-10-31 发布日期:2019-09-18
通讯作者: Tel.: +86-514-87992215, E-mail: prhurong@163.com; bcyan@yzu.edu.cn
基金资助:
The University Natural Science Research major Project of Jiangsu Province (Grant No. 16KJA310006).

Porcine placental peptides improve neuroblast proliferation and differentiation via enhancement of TrkB and BDNF levels in D-glatacose-induced mouse aging model

Xu Quan¹, Caihong Gu³, Feng Yan⁴, Yaoyao Gao⁵, Lin Chen⁶, Rong Wei⁷, Bingchun Yan^1,2*, Rong Hu^1,2*

1. Department of Pharmacy, Institute of Translational Medicine, Medical College, Yangzhou University,Yangzhou 225001, Jiangsu, China
2. The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou University, Yangzhou 225001, Jiangsu, China
3. Jiangsu Lianhuan Pharmaceutical Group Co., Ltd., Yangzhou 225009, Jiangsu, China
4. Jiangsu Yangnong Chemical Group Co., Ltd., Yangzhou 225001, Jiangsu, China
5. Gaoyou Hospital of Traditional Chinese Medicine, Yangzhou University, Yangzhou 225600, Jiangsu, China
6. Tongzhou District People's Hospital, Nantong 226300, Jiangsu, China
7. Nantong Tumor Hospital, Nantong 226361, Jiangsu, China

Received:2019-06-30 Revised:2019-08-27 Online:2019-10-31 Published:2019-09-18
Contact: Tel.: +86-514-87992215, E-mail: prhurong@163.com; bcyan@yzu.edu.cn
Supported by:
The University Natural Science Research major Project of Jiangsu Province (Grant No. 16KJA310006).

摘要/Abstract

摘要：

本研究旨在探讨猪胎盘肽抗神经老化的药理作用和机制,观察其对D-半乳糖老化模型小鼠海马齿状回神经母细胞的增殖和分化的影响及其相关蛋白表达的变化。在本实验中,我们发现猪胎盘肽对D-半乳糖诱导的衰老小鼠具有抵抗神经老化的作用,能够明显上调DCX免疫反应的神经母细胞的数量、同时, BDNF和TrkB的表达明显上调,抗凋亡蛋白Bcl-2的表达明显上调,促凋亡蛋白Caspase3、8、9的表达明显下降。本研究结论表明猪胎盘肽具有明显的抗神经老化作用,其抵抗作用机制与调控Bcl-2、Caspase3、8、9等调网相关蛋白及BDNF/TrkB通路密切相关。本研究为猪胎盘肽在保健和医药领域的应用提供了理论依据。

关键词: 猪胎盘肽, 抗衰老, 神经母细胞增殖和分化, 凋亡相关蛋白, BDNF/TrkB信号通路

Abstract:

Aging affects the nervous system as well as other organs. In our study, we aimed to study the pharmacological effects and mechanism of porcine placental peptides (PPP) in aging process, and to observe the changes of neuroblast proliferation and differentiation as well as partial gene expression in hippocampus of D-galactose-induced aged mouse. Based on the analysis of experimental results, it was confirmed that PPP significantly improved neurobalst proliferation and differentiation in the mouse hippocampal DG by ki-67 and DCX immunohistochemistry. This result showed that PPP had anti-aging effects on D-galactose-induced aging mouse model. Moreover, we observed up-regulated expressions of BDNF and TrkB proteins and down-regulated expressions of Caspase 3, 8, and 9 proteins in the PPP-treated mouse hippocampus. Therefore, our results showed that PPP obviouslyimproved neuroblast proliferation and differentiation, and its anti-aging effect might berelated to down-regulation of apoptosis-related proteins, including Caspase 3, 8, and 9, via BDNF/TrkB pathway. Our findings provided valuable evidence for its applications in the health and medicine sectors.

Key words: Porcine placenta peptide, Anti-aging, Neuroblast proliferation and differentiation, Apoptosis-related proteins, BDNF/TrkB pathway

中图分类号:

R963

Supporting:

权栩, 顾彩虹, 严峰, 高垚垚, 陈琳, 卫榕, 颜丙春, 胡荣. 猪胎盘肽通过提高TrkB和BDNF水平及促进神经母细胞增殖和分化延缓D-半乳糖诱导的小鼠衰老[J]. 中国药学(英文版), 2019, 28(10): 739-748.

Xu Quan, Caihong Gu, Feng Yan, Yaoyao Gao, Lin Chen, Rong Wei, Bingchun Yan, Rong Hu. Porcine placental peptides improve neuroblast proliferation and differentiation via enhancement of TrkB and BDNF levels in D-glatacose-induced mouse aging model[J]. Journal of Chinese Pharmaceutical Sciences, 2019, 28(10): 739-748.

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猪胎盘肽通过提高TrkB和BDNF水平及促进神经母细胞增殖和分化延缓D-半乳糖诱导的小鼠衰老

Porcine placental peptides improve neuroblast proliferation and differentiation via enhancement of TrkB and BDNF levels in D-glatacose-induced mouse aging model

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