中国药学(英文版) ›› 2023, Vol. 32 ›› Issue (7): 560-573.DOI: 10.5246/jcps.2023.07.047
收稿日期:
2022-12-05
修回日期:
2023-01-14
接受日期:
2023-02-21
出版日期:
2023-07-31
发布日期:
2023-07-31
通讯作者:
杨秀伟
作者简介:
基金资助:
Yanfang Yang, Wei Wei, Lei Zhang, Wei Xu, Xiuwei Yang*()
Received:
2022-12-05
Revised:
2023-01-14
Accepted:
2023-02-21
Online:
2023-07-31
Published:
2023-07-31
Contact:
Xiuwei Yang
摘要:
都梁丸是中药经典方剂, 由白芷和川芎组成。为了研究都梁丸的药效物质基础, 我们采用Diamonsil C18柱、乙腈和0.05%甲酸水溶液为流动相、电喷雾电离源和多重反应监测模式, 建立了同时测定白芷、川芎中37种成分的UPLC-MS/MS法并进行了方法学验证。同时, 我们采用该方法测定了口服都梁丸提取物后的大鼠体内药代动力学, 发现这37种成分均能迅速吸收入血, 大部分化合物在24小时内被完全清除, 白芷香豆素的口服吸收效果优于川芎内酯类化合物, 都梁丸中白芷香豆素的清除速度明显慢于白芷单一给药。该结果为都梁丸的药效物质基础和白芷-川芎配伍作用机制研究提供了依据。
Supporting:
杨雁芳, 韦玮, 张雷, 徐嵬, 杨秀伟. UPLC-MS/MS法测定都梁丸中37个成分在大鼠体内的药代动力学[J]. 中国药学(英文版), 2023, 32(7): 560-573.
Yanfang Yang, Wei Wei, Lei Zhang, Wei Xu, Xiuwei Yang. Simultaneous quantification of 37 constituents in Duliang Pill by UPLC-MS/MS: Application to the pharmacokinetic studies in rat plasma[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(7): 560-573.
Figure 1. Chemical structures of primary components from DLP. (1) Osthenol, (2) dehydrogeijerin, (3) 7-demethylsuberosin, (4) psoralen, (5) bergapten, (6) xanthotoxol, (7) xanthotoxin, (8) 5-hydroxy-8-methoxypsoralen, (9) isopimpinellin, (10) isodemethylfuropinarine, (11) alloimperatorin, (12) alloisoimperatorin, (13) imperatorin, (14) phellopterin, (15) isoimperatorin, (16) heraclenol, (17) byakangelicin, (18) heracol, (19) isooxypeucedanin, (20) oxypeucedanin hydrate, (21) oxypeucedanin, (22) oxypeucedanin ethanolate, (23) oxypeucedanin methanolate, (24) isogosferol, (25) 2??R-neobyakangelicol, (26) byakangelicol, (27) isobyakangelicol, (28) (2?S,3?R)-3?-hydroxymarmesinin, (29) senkyunolide I, (30) senkyunolide H, (31) senkyunolide G, (32) senkyunolide A, (33) Z-ligustilide, (34) 3-butylidenephthalide, (35) neocnidilide, (36) cnidilide, and (37) levistolide A.
Figure 2. MRM results of the analytes (A. blank plama; B. blank plama spiked with analytes and IS; C. plasma sample at 2 h after oral administration of DLP).
Table 2. Intra- and inter-day precisions and accuracies, recoveries, stabilities, and matrix effects of the 37 analytes in rat plasma (mean ± SD, n = 6).
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