高温胁迫对防风药材药代动力学和药效的影响

doi:10.5246/jcps.2018.02.012

中国药学(英文版) ›› 2018, Vol. 27 ›› Issue (2): 109-115.DOI: 10.5246/jcps.2018.02.012

高温胁迫对防风药材药代动力学和药效的影响

姜华^1,2, 杨景明², 曹玲¹, 贾桂枝³, 戴红良⁴, 孟祥才^1*

1. 黑龙江中医药大学, 黑龙江哈尔滨 150040
2. 锦州医科大学药学院, 辽宁锦州 121000
3. 锦州医科大学基础医学院, 辽宁锦州 121001
4. 锦州医科大学护理学院, 辽宁锦州 121001

收稿日期:2017-12-13 修回日期:2018-01-27 出版日期:2018-03-03 发布日期:2018-02-12
通讯作者: Tel.: +86-451-87266802, E-mail: mengxiangcai000@163.com
基金资助:
National Natural Science Foundation of China (Grant No. 81541079), Natural Science Foundation of Heilongjiang province (Grant No. H2017065) and the Doctoral Scientific Research Foundation of Liaoning Province (Grant No. 20170520132).

Quality evaluation of heat stress treated Radix saposhnikoviae using pharmacokinetic and pharmacologic methods

Hua Jiang^1,2, Jingming Yang², Ling Cao¹, Guizhi Jia³, Hongliang Dai⁴, Xiangcai Meng^1*

1. College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China
2. College of Pharmacy, Jinzhou Medical University, Jinzhou 121001, China
3. Department of Physiology, Jinzhou Medical University, Jinzhou 121001, China
4. School of Nursing, Jinzhou Medical University, Jinzhou 121001, China

Received:2017-12-13 Revised:2018-01-27 Online:2018-03-03 Published:2018-02-12
Contact: Tel.: +86-451-87266802, E-mail: mengxiangcai000@163.com
Supported by:
National Natural Science Foundation of China (Grant No. 81541079), Natural Science Foundation of Heilongjiang province (Grant No. H2017065) and the Doctoral Scientific Research Foundation of Liaoning Province (Grant No. 20170520132).

摘要/Abstract

摘要：

以防风鲜根为研究材料, 研究高温环境胁迫对药材主要活性成分色原酮的药代动力学参数和药效的影响。采用HPLC法测定灌胃给药40 ºC高温处理3天后防风和普通防风中升麻素、升麻苷、5-O-甲基维斯阿米醇苷三种色原酮成分药代动力学的变化, 并以肛温法、热板法、足跖法进一步研究两组药物解热、镇痛和抗炎三项指标药效强度差异。结果显示两组防风大鼠血浆中只检测到升麻素成分, 但40 ºC高温胁迫组的AUC_0–_{24 h}比普通防风增加了50.6%, 并且解热、镇痛和抗炎药效均增强。因此高温胁迫可显著提高防风的解热、镇痛和抗炎等药理作用, 是提高防风药材质量的途径。

关键词: 防风, 色原酮, 药代动力学, 解热作用, 镇痛作用, 抗炎作用

Abstract:

The quality of Radix saposhnikoviae (RS) had deteriorated due to the adoption of cultivated plants rather than wild plants. High temperature treatment increases the content of several chromones, however using one or more individual constituents would be difficult to determine the medical quality of RS. In this paper, we used pharmacokinetic and pharmacologic approaches to evaluate the quality of RS. The active constituents were analysed using pharmacokinetic parameters of the chromone derivatives, and the antipyretic, analgesic, and anti-inflammatory effects were evaluated by pyretic animal model, hot plate test, and ear edema model, respectively. Only cimifugin was found in plasma after RS and heat-stress-RS were administered to rats, with a 50.6% increase in AUC_0–_{24 h} of cimifugin in the latter. Likewise, more potent anti-pyretic, analgesic, and anti-inflammatory activities were also found in the latter. Exposure of S. divaricata fresh roots to high temperatures enhanced the antipyretic, analgesic, and anti-inflammatory effects of RS by promoting the absorption of cimifugin.

Key words: Radix Saposhnikoviae, Chromone, Pharmacokinetics, Anti-pyretic, Analgesic, Anti-inflammatory

中图分类号:

R969.1

Supporting:

姜华, 杨景明, 曹玲, 贾桂枝, 戴红良, 孟祥才. 高温胁迫对防风药材药代动力学和药效的影响[J]. 中国药学(英文版), 2018, 27(2): 109-115.

Hua Jiang, Jingming Yang, Ling Cao, Guizhi Jia, Hongliang Dai, Xiangcai Meng. Quality evaluation of heat stress treated Radix saposhnikoviae using pharmacokinetic and pharmacologic methods[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(2): 109-115.

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高温胁迫对防风药材药代动力学和药效的影响

Quality evaluation of heat stress treated Radix saposhnikoviae using pharmacokinetic and pharmacologic methods

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