芒果产业废弃物中快速提取高纯度芒果苷的研究

doi:10.5246/jcps.2021.10.071

中国药学(英文版) ›› 2021, Vol. 30 ›› Issue (10): 831-837.DOI: 10.5246/jcps.2021.10.071

芒果产业废弃物中快速提取高纯度芒果苷的研究

庞朝海¹^,², 田海¹^,², 郭晓杰³, 王盼³, 韩丙军¹^,², 阳辛凤¹^,²^,^*()

1. 中国热带农业科学院分析测试中心, 海南海口 571101
2. 海南省热带果蔬产品质量安全重点实验室, 海南海口 571101
3. 海南大学热带作物学院, 海南海口 570228

收稿日期:2021-02-15 修回日期:2021-04-21 接受日期:2021-05-15 出版日期:2021-10-24 发布日期:2021-10-24
通讯作者: 阳辛凤
作者简介:
+ Tel.: +86-898-66895007, E-mail: yangxinf@sina.com; 18389859589@163.com
基金资助:
The Key R & D projects in Hainan Province (Grant No. ZDYF2020160), the National Key Research and Development Project of China (Grant No. 2017YFF0207800) and Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (Grant No. 1630082017001).

Facile extraction of high-purity mangiferin from mango industrial wastes

Chaohai Pang¹^,², Hai Tian¹^,², Xiaojie Guo³, Pan Wang³, Bingjun Han¹^,², Xinfeng Yang¹^,²^,^*()

1 Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China
2 Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Haikou 571101, Hainan, China
3 College of Tropical Crops, Hainan University, Haikou 570228, Hainan, China

Received:2021-02-15 Revised:2021-04-21 Accepted:2021-05-15 Online:2021-10-24 Published:2021-10-24
Contact: Xinfeng Yang

摘要/Abstract

摘要：

本研究描述了一种从新鲜芒果叶片中分离纯化天然芒果苷的新颖简单且可大量制备的方法。以甲醇作为溶剂, 采用超声辅助的方法从芒果叶片中提取芒果苷。芒果苷粗提物以及通过甲醇–氯仿 (10:1, v/v)重结晶得到的产物的得率分别为1.41% (纯度86.5%) 和0.75% (纯度大于99.6%)。采用紫外分光光度法和高效液相法对芒果苷进行纯度分析。通过UV、IR、Q-TOF/MS、¹H NMR和¹³C NMR对纯化后的化合物结构进行鉴定。本文报道了一种简单、经济、快速、高效的芒果苷纯化方法。同时, 采用该方法首次获得纯度超过99.6%的芒果苷。

关键词: 芒果叶, 芒果苷, 提取, 重结晶, 纯度分析

Abstract:

In the present study, we described a novel, simple, and scalable method for isolating and purifying natural mangiferin from fresh mango leaves. An ultrasonic-assisted method was used to extract mangiferin from mango leaves using methanol as the solvent. The yields of the mangiferin crude extract and purified mangiferin recrystallized from a mixture of methanol and trichloromethane (10:1, v/v) were 1.41% (purity 86.5%) and 0.75% (purity > 99.6%), respectively. The purity of mangiferin was assessed by UV spectroscopy and HPLC. The structure of the purified compound was confirmed spectroscopically by UV, IR, Q-TOF/MS,¹H NMR, and¹³C NMR. The method described herein to isolate mangiferin was simple, inexpensive, rapid, and efficient. Moreover, we, for the first time, obtained mangiferin with a purity of more than 99.6% using this method.

Key words: Mango leaves, Mangiferin, Extraction, Recrystallization, Purity analysis

Supporting:

庞朝海, 田海, 郭晓杰, 王盼, 韩丙军, 阳辛凤. 芒果产业废弃物中快速提取高纯度芒果苷的研究[J]. 中国药学(英文版), 2021, 30(10): 831-837.

Chaohai Pang, Hai Tian, Xiaojie Guo, Pan Wang, Bingjun Han, Xinfeng Yang. Facile extraction of high-purity mangiferin from mango industrial wastes[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(10): 831-837.

图/表 5

Figure 1. Structure of mangiferin.

Figure 2. HPLC chromatograms: (A) mangiferin crude extract (2 μg/mL), purified mangiferin (10 μg/mL) and solvent blank; (B) HPLC chromatograms of purified mangiferin at different detection wavelengths.

Figure 3. (a) HPLC chromatograms of purified mangiferin at different chromatographic columns: Waters Xbridge BEH C18; (b) HPLC chromatograms of purified mangiferin at different chromatographic columns: Agilent ZORBAX SB-Aq.

Figure 4. (a) HPLC chromatograms of purified mangiferin at different elution conditions: gradient elution mode; (b) HPLC chromatograms of purified mangiferin at different elution conditions: isocratic elution mode.

Table 1. Results of HPLC analysis under different conditions.

参考文献 18

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芒果产业废弃物中快速提取高纯度芒果苷的研究

Facile extraction of high-purity mangiferin from mango industrial wastes

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