气-质联用分析比较麻黄及其炮制品中挥发性成分

气-质联用分析比较麻黄及其炮制品中挥发性成分

钟凌云^*, 龚千锋, 祝婧, 陈芳

江西中医学院药学院, 江西南昌 330004

收稿日期:2009-09-29 修回日期:2009-11-15 出版日期:2010-01-15 发布日期:2010-01-15
通讯作者: 钟凌云*

Comparative analysis of essential oil components in Ephedra and its processed products by GC-MS

Ling-Yun Zhong^*, Qian-Feng Gong, Jing Zhu, Fang Chen

Pharmaceutical College, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China

Received:2009-09-29 Revised:2009-11-15 Online:2010-01-15 Published:2010-01-15
Contact: Ling-Yun Zhong*

摘要/Abstract

摘要： 采用气质联用法分析麻黄生品及其炮制品种的挥发油成分。采取水蒸汽蒸馏法和超临界流体萃取技术提取麻黄各品种中的挥发油, 其中通过水蒸汽蒸馏法提取得到的麻黄生品、蜜麻黄和清炒麻黄挥发油中, 鉴定的成分分别为48, 57和48种, 蜜炙和清炒后分别产生14 和9种新成分; 通过超临界流体萃取法提取得到的麻黄生品、蜜麻黄和清炒麻黄挥发油中, 鉴定的成分分别为22, 36和28 种, 蜜炙和清炒后分别产生15 和23种新成分。麻黄经炮制后挥发油成分和相对含量均发生了明显变化, 这可能是导致麻黄炮制品功效的改变原因之一。

关键词: 挥发油, 炮制, 气质联用

Abstract: The similarities and differences of essential oil components in the raw ingredients of Ephedra (RIE) and processed products of Ephedra obtained by stir-baking with honey or stir-baking without any supplements were analyzed by GC-MS. Wet distillation (WD) and supercritical fluid extraction (SFE) were used to extract essential oil components from the samples. Total 48, 57 and 48 compounds were found in the extracted essential oils using WD from RIE, the products obtained by stir-baking with honey from Ephedra (SBHE) and the products obtained by stir-baking without any supplements from Ephedra (SBE), respectively; whereas total 22, 36 and 28 compounds were identified in the extracted essential oils using SPE from these three samples, respectively. In addition, 14 and 9 new compounds were found in the essential oils extracted using WD from SBHE and SBE, whereas 15 and 23 new compounds were found in the essential oils extracted by SFE from SBHE and SBE, respectively. The composition and concentration of the essential oil components in the processed products were significantly different from RIE. Such changes in essential oil components might affect drug actions, which is dependent on the manner in which the sample is processed. The findings in this study may shed some lights on the understanding and further exploration of Ephedra processing.

Key words: Ephedra essential oil, Ephedra processing, GC-MS

中图分类号:

R917.4

Supporting:

Foundation item: State Projects of the Eleventh-Five-year Plan (Grant No. 2006BAI09B06-08-05).
^*Corresponding author. Tel.: 13870890930

钟凌云^*, 龚千锋, 祝婧, 陈芳. 气-质联用分析比较麻黄及其炮制品中挥发性成分[J]. .

Ling-Yun Zhong^*, Qian-Feng Gong, Jing Zhu, Fang Chen . Comparative analysis of essential oil components in Ephedra and its processed products by GC-MS [J]. .

参考文献

[1] Gong, Q.F. The Processing of Chinese Materia Medica. Beijing: China Press of Traditional Chinese Medicine. 2007, 231-233.
[2] Yin, J.; Guo, L.G. Contemporary Study and Clinical application of Traditional Chinese Medicine. Beijing: Xueyuan Press. 1995, 612-622.
[3] Chinese Pharmacopia. Beijing: Chemical Industry Publishment. 2005, 223-224.
[4] Gong, Q.F. The Processing of the Traditional Chinese Medicine of Zhangshu. Nanchang: Jiangxi Science and Technology Press. 1991, 182-183.
[5] Ji, L.; Xu, Z.L.; Pan, J.G. Chin. J. Chin. Mater. Med. 1997, 22, 489-491.
[6] Zhang, J.C. Supercritical Fluid Extraction. Beijing: Chemical Industry Press. 2000, 112-113.
[7] Yang, P.; Han, L.J.; Zhang, L.W. Chem. Res. Appl. 2001, 13, 128-130.
[8] Liao, C.H.; Huang, Z.R. The Development and Application of Supercritical Fluid Extraction. Beijing: Chemical Industry Press. 2004, 7-9.
[9] Yang, Y.; Qu, Y.Q.; Shu, S.L. J. Jiangsu Univ (Medicine Edition). 2007, 17, 394-396.
[10] Chen, K.; Xu, X.F.; Lin, L.; Lin, W.J. Shizhen Med. Mat. Med. Res. 2005, 16, 465-466.
[11] Gong, F.; Liang, Y.Z.; Cui, H. J. Chromatogr. A. 2001, 909, 237-239.
[12] Gong, F.; Liang, Y.Z.; Xu, Q.S. J. Chromatogr. A. 2001, 905, 193-195.
[13] Li, X.R.; Lan, Z.G.; Liang, Y.Z. J. Central South Univ (English edition). 2007, 14, 57-59.
[14] Shao, H.W.; Zhu, W.P. China Pharm. 2006, 15, 32-35.
[15]Wang, W.; Zhao, Z.L.; Niu, Z.D. Chin. Tradit. Herb. Drugs. 2003, 34, 506-507.
[16] Wang, W.J. Chin. Food Additives. 2005, 1, 133-135.
[17] Zhang, C.Y.; Yu, J.; Wang, B.; Cai, S.Q. Chin. J. Nat. Med. 2004, 2, 116-118.
[18] Zeng, Q.; Xian, Q.M. Chin. Tradit. Herb. Drugs, 1997, 28, 81-84.
[19] Li, H.Q.; Gong, J.X. Textile J. 2002, 2, 50-53.
[20] Zhang, J. Dye. Fin.2006, 32, 244-246.
[21] Liu, Y.F.; Wang, Y.X.; Wu, B. J. Pharm. Sci. 2006, 15, 38-40.
[22] Gao, Y.; Chen, H.J.; Qian, L.H. J. Appl. Clin. Pediatr. 2006, 21, 361-363.