白芍和白术配伍麸炒前后主要化学成分变化HPLC分析

doi:10.5246/jcps.2015.04.030

中国药学(英文版) ›› 2015, Vol. 24 ›› Issue (4): 231-235.DOI: 10.5246/jcps.2015.04.030

白芍和白术配伍麸炒前后主要化学成分变化HPLC分析

方新华¹, 吴鑫², 曹岗^2,3*, 蔡皓³, 蔡宝昌^2,3*

1. 杭州市中医院中药房, 浙江杭州 310007
2. 浙江中医药大学中药炮制技术研究中心, 浙江杭州 311401
3. 南京中医药大学药学院, 江苏南京 210023

收稿日期:2015-02-06 修回日期:2015-03-11 出版日期:2015-04-28 发布日期:2015-03-15
通讯作者: Tel.: 86-571-87195895
基金资助:
The National Natural Science Foundation of China (Grant No. 81202918), the Open Project of National First-Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine (Grant No. 2011ZYX2-006), the Science and Technology Project of Hangzhou, China (Grant No. 20130533B68 and 20131813A23), the Chinese Medicine Research Program of Zhejiang Province, China (Grant No. 2014ZQ008 and 2015ZQ011), and the Science Foundation of Zhejiang Chinese Medical University (Grant No. 2011ZY25 and 2013ZZ12).

Analysis of the influence of processing of bran stir-baking on the main components of Paeoniae Radix Alba-Atractylodis Macrocephalae Rhizoma herbal pair by high-performance liquid chromatography

Xinhua Fang¹, Xin Wu², Gang Cao^2,3*, Hao Cai³, Baochang Cai^2,3*

1. TCM Dispensary, Traditional Chinese Medical Hospital of Hangzhou, Hangzhou 310007, China
2. Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 311401, China
3. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China

Received:2015-02-06 Revised:2015-03-11 Online:2015-04-28 Published:2015-03-15
Contact: Tel.: 86-571-87195895
Supported by:
The National Natural Science Foundation of China (Grant No. 81202918), the Open Project of National First-Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine (Grant No. 2011ZYX2-006), the Science and Technology Project of Hangzhou, China (Grant No. 20130533B68 and 20131813A23), the Chinese Medicine Research Program of Zhejiang Province, China (Grant No. 2014ZQ008 and 2015ZQ011), and the Science Foundation of Zhejiang Chinese Medical University (Grant No. 2011ZY25 and 2013ZZ12).

摘要/Abstract

摘要：

白芍和白术配伍起源于白术芍药散, 临床上其生品配伍与制品配伍用于治疗不同的疾病。为了评价白芍和白术生品配伍和制品配伍之间的区别, 本实验选取了简单, 易行且灵敏度高的HPLC-DAD法同时测定其中9种活性成分的变化。线性关系良好, r²≥0.9992。检测限和定量限分别小于7.30 µg/mL和11.53 µg/mL。日内、日间精密度及重复性RSD值分别小于3.86%、2.71%和4.29%。稳定性RSD值小于3.64%。加样回收率在96.70%–102.10%, RSD值小于3.52%。建立的方法可以用于白芍和白术配伍内在质量的控制。

关键词: 高效液相色谱, 炮制, 定量, 白芍, 白术

Abstract:

The crude and processed Paeoniae Radix Alba-Atractylodis Macrocephalae Rhizoma herbal pairs, originated from Bai-zhu-shao-yao-san, are used to treat different diseases clinically. In order to evaluate the crude and processed Paeoniae Radix-Atractylodis MacrocephalaeRhizoma herbal pairs, a simple, easy, and sensitive high-performance liquid chromatography coupled with diode array detectors was developed for simultaneous determination of nine bioactive components in the herbal pairs. The calibration curve exhibited good linearity (r²≥0.9992). The LODs and LOQs were ≤7.30 and 11.53 µg/mL, respectively. The intra-, inter-day and repeatability RSD values of the nine compounds were less than 3.86%, 2.71%, and 4.29%, respectively. The RSD stability values were less than 3.64%. The recovery of the method was in the range of 96.70%–102.10%, with RSD values less than 3.52%. The developed method can be applied to the intrinsic quality control of crude and processed Paeoniae Radix-Atractylodis Macrocephalae Rhizoma herbal pairs.

Key words: High-performance liquid chromatography, Processing, Quantification, Paeoniae Radix Alba, Atractylodis Macrocephalae Rhizoma

中图分类号:

R284

Supporting:

方新华, 吴鑫, 曹岗, 蔡皓, 蔡宝昌. 白芍和白术配伍麸炒前后主要化学成分变化HPLC分析[J]. 中国药学(英文版), 2015, 24(4): 231-235.

Xinhua Fang, Xin Wu, Gang Cao, Hao Cai, Baochang Cai. Analysis of the influence of processing of bran stir-baking on the main components of Paeoniae Radix Alba-Atractylodis Macrocephalae Rhizoma herbal pair by high-performance liquid chromatography[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(4): 231-235.

参考文献

[1] Cui, J.Z. Inner. Mongol. J. Tradit. Chin. Med. 2013, 32, 43-44.

[2] Zhou, J.L.; Qi, L.W.; Li, P. J. Chromatogr. A. 2009, 1216, 7582-7594.

[3] Li, P.; Qi, L.W.; Liu, E.H.; Zhou, J.L.; Wen, X.D. Trends Anal. Chem. 2008, 27, 66-77.

[4] Zhou, S.S.; Xu, J.D.; Zhu, H.; Shen, H.; Xu, J.; Mao, Q.; Li, S.L.; Yan, R. Molecules. 2014, 19, 4008-4104.

[5] Cai, H.; Xu, Z.W.; Luo, S.C.; Zhang, W.W.; Cao, G.; Liu, X.; Lou, Y. J.; Ma, X.Q.; Qin, K.M.; Cai, B.C. Pharmacogn. Mag. 2012, 8, 300-307.

[6] Cao, G.; Cai, H.; Zhang, Y.; Cong, X.D.; Zhang, C.R.; Cai, B.C. J. Pharm. Biomed. Anal. 2011, 56, 118-125.

[7] Hu, J.; Wu, X.; Cao, G.; Chen, X.C. Nat. Prod. Res. 2014, 21, 1853-1858.

[8] Cao, G.; Li, Q.L.; Cai, H.; Tu, S.C.; Cai, B.C. Evid. Based Compl. Alt. Med. 2014, 2014, 1-14.

[9] Zhang, J.J.; Huang, Y.F.; Wang, L.L.; Li, W.; Wang, J.X.; Wang, C.; Qu, S.S. Chin. J. Chin. Mater. Med. 2013, 38, 3358-3362.

[10] Zhang, J.D.; Cai, H.; Cao, G.; Liu, X.; Wen, C.P.; Fan, Y.S. Evid. Based Compl. Alt. Med. 2013, 2013, 1-9.

[11] Wu, D.; Chen, J.; Zhu, H.; Xiong, X.G.; Liang, Q.H.; Zhang, Y.; Zhang, Y.; Wang, Y.; Yang, B.; Huang, X. Exp. Ther. Med. 2014, 7, 209-217.

[12] Cao, G.; Cai, H.; Jiang, J.P.; Yao, L.J.; Tu, S.C.; Wang, L.; Ma, X.Q.; Cai, B.C. J. Sep. Sci. 2014, 37, 1194-1198.

[13] Cui, X.B.; Shan, C.X.; Wen, H.M.; Li, W.; Wu, H. Chin. J Chin. Mater. Med. 2013, 38, 1929-1933.

[14] Bose, S.; Kim, H. Evid. Based Compl. Alt. Med. 2013, 2013, 1-16.

[15] Wu, X.; Cao, G.; Li, Q.L.; Cai, B.C.; Hu, S.Y. J. Sep. Sci. 2015, 38, 239-246.

[16] Zheng, L.; Shao, Z.D.; Wang, Z.C.; Fu, C.X. Int. J. Mol. Sci. 2012, 13, 16046-16052.

[17] Guo, L.; Sun, Y.L.; Wang, A.H.; Xu, C.E.; Zhang, M.Y. Mol. Biol. Rep. 2012, 39, 9285-9290.

[18] Liu, S.Y.; Xue, D.S.; Pan, J.C.; Pan, J.C.; Zhang, W.M.; Li, W.D.; Qu, H.B. Chin. J. Nat. Med. 2014, 12, 535-541.

[19] Yang, Y.; Yin, X.J.; Guo, H.M.; Wang, R.L.; Song, R.; Tian, Y.; Zhong, C.J. Chin. J. Nat. Med. 2014, 12, 542-553.

[20] Sun, S.; Xie, Z.S.; Liu, E.H.; Yang, Y.T.; Xu, X.J.; Li, P. Chin. J. Nat. Med. 2014, 12, 229-240.

[21] Zhao, W.L.; Wu, H.; Shan, G.S.; Jia, T.Z. Chin. J. Chin. Mater. Med. 2013, 38, 3493-3497.

白芍和白术配伍麸炒前后主要化学成分变化HPLC分析

Analysis of the influence of processing of bran stir-baking on the main components of Paeoniae Radix Alba-Atractylodis Macrocephalae Rhizoma herbal pair by high-performance liquid chromatography

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