大蒜辣素对人鼻咽癌KB细胞的促凋亡、周期阻滞以及微管解聚作用

大蒜辣素对人鼻咽癌KB细胞的促凋亡、周期阻滞以及微管解聚作用

杨亚平, 李敏^*, 徐波, 郭维, 崔景荣, 王夔

1. 北京大学天然药物及仿生药物国家重点实验室, 北京 100191
2. 中亚地区可食植物功能成分联合实验室北京大学分部, 北京 100191

收稿日期:2009-02-13 修回日期:2009-05-10 出版日期:2009-06-15 发布日期:2009-06-15
通讯作者: 李敏*

Allicin induces apoptosis, cell cycle arrest and microtubule disassembly in human nasopharyngeal carcinoma KB cells

Ya-Ping Yang, Min Li^*, Bo Xu, Wei Guo, Jing-Rong Cui, Kui Wang

1. State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China
2. Union Laboratories of Bioactive Ingredients of Edible Plants from Central Asia, Peking University Division, Beijing 100191, China

Received:2009-02-13 Revised:2009-05-10 Online:2009-06-15 Published:2009-06-15
Contact: Min Li*

摘要/Abstract

摘要： 大蒜辣素是大蒜的主要生物活性成分, 由其非活性前体蒜氨酸与蒜酶相互作用而产生。在此项研究中, 我们观测大蒜辣素对人鼻咽癌细胞的增殖抑制作用与药理机制。研究结果表明, 大蒜辣素可明显抑制KB细胞的增殖, 具有浓度依赖性, 作用48 h的IC50值为(2.2±0.2) μg/mL。而且, 大蒜辣素可诱导KB细胞凋亡, 作用48 h时诱导凋亡作用在(16~48) μg/mL范围内具有浓度依赖性。大蒜辣素的凋亡诱导作用通过其诱导KB细胞的细胞核凝集、细胞骨架改变以及线粒体膜电势变化得到了进一步确证。大蒜辣素还可阻滞KB细胞周期于S期, 在(16~48) μg/mL范围内具有浓度依赖性。此外, 大蒜辣素可浓度依赖的解聚KB细胞的微管, 其作用类似于已知的微管解聚剂秋水仙碱。大蒜辣素对人鼻咽癌KB细胞的增殖抑制作用可能是通过诱导凋亡, 阻滞细胞周期以及解聚微管实现的。

关键词: 大蒜辣素, 鼻咽癌, 凋亡, 细胞周期, 微管, 高内涵筛选分析

Abstract: Allicin, a major biologically active component of garlic, is produced from its inactive precursor alliin by the enzyme alliinase. In this study, we investigated its effects on human nasopharyngeal carcinoma KB cells. After incubation for 48 h, allicin inhibited the growth of KB cells in a concentration-dependent manner with an IC50value of (2.2±0.2) μg/mL. Incubation with allicin for 48 h caused a concentration-dependent induction of apoptosis in the concentration range of (16–48) μg/mL, and the induction of apoptosis was confirmed by the changes of mitochondrial membrane potential, F-actin contents and nuclear condensation in KB cells. Moreover, allicin concentration-dependently arrested KB cells at the S-phase of the cell cycle in the range of (16–48) μg/mL. In addition, treatment with the compound caused concentration-dependent disassembly of microtubule cytoskeleton in KB cells, which is similar to the effect of colchicine, a well-known microtubule destabilizing agent. We concluded that the abilities of allicin to inhibit the proliferation of KB cells probably relate to its apoptosis induction, cell cycle arrest and microtubule destabilizing properties

Key words: Allicin, Allicin, Nasopharyngeal carcinoma, Nasopharyngeal carcinoma, Apoptosis, Apoptosis, Cell cycle, Cell cycle, Microtubule, Microtubule, High content screening, High content screening

中图分类号:

R961

Supporting:

Foundation items: Grant of Modernization and Industrization of Weiur Medicine (Grant No. 2007BAI30B02-4), the grant from State Key Laboratory of Natural and Biomimetic Drugs, Peking University.
^*Corresponding author. Tel.: 86-10-82801161; e-mail: limin@bjmu.edu.cn

杨亚平, 李敏^*, 徐波, 郭维, 崔景荣, 王夔. 大蒜辣素对人鼻咽癌KB细胞的促凋亡、周期阻滞以及微管解聚作用[J]. .

Ya-Ping Yang, Min Li^*, Bo Xu, Wei Guo, Jing-Rong Cui, Kui Wang. Allicin induces apoptosis, cell cycle arrest and microtubule disassembly in human nasopharyngeal carcinoma KB cells [J]. .

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