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高效液相色谱法测定大鼠血浆中吉马酮

何海冰, 唐星*, 崔福德   

  1. 沈阳药科大学药学院, 辽宁 沈阳 110016
  • 收稿日期:2004-03-30 修回日期:2004-08-10 出版日期:2004-09-15 发布日期:2004-09-15
  • 通讯作者: 唐星*

High-Performance Liquid Chromatographic Method for Determination of Germacrone in Rat Plasma

HE Hai-bing, TANG Xing*, CUI Fu-de   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2004-03-30 Revised:2004-08-10 Online:2004-09-15 Published:2004-09-15
  • Contact: TANG Xing*

摘要: 目的 建立莪术挥发油中吉马酮在大鼠血浆中的高效液相色谱测定方法. 方法 血浆样品经乙腈沉淀蛋白处理后, 取上清液在244nm波长下采用高效液相色谱法进行分析. 结果 吉马酮在血中的检测限为100ng·mL-1, 线性范围为0.1004-15.06ug·mL-1.日内和日间的RSD分别为1.87%-4.29%1.29%-5.15%.吉马酮的提取回收率为95%以上. 血浆中的内源性物质对测定没有影响. 结论 该法操作简便、快速,适用于吉马酮在体内的药代动力学研究.

关键词: 高效液相色谱法, 高效液相色谱法, 高效液相色谱法, 吉马酮, 吉马酮, 吉马酮, 莪术油, 莪术油, 莪术油

Abstract: Aim A reliable high-performance liquid chromatographic (HPLC) method was developed for determination of germacrone in rat plasma. Methods The plasma samples were treated with acetonitrile and analyzed by HPLC with UV detection at 244 nm. Results The limit of detection was 100 ng·mL-1 for germacrone in plasma and the linear range was 0.100 4-15.06 μg·mL-1 in plasma. The RSD of intra-day and inter-day assay was 1.87%-4.29% and 1.29%-5.15%, respectively. The recoveries of germacrone were over 95%. The endogenous substances in plasma did not show any interference in the analysis. Conclusion The method is accurate and convenient, and suitable for pharmacokinetic studies of germacrone in rats.

Key words: high-performance liquid chromatography, high-performance liquid chromatography, germacrone, germacrone, volatile oil of Rhizoma Curcumae, volatile oil of Rhizoma Curcumae

Supporting: *Corresponding author. Tel.: 86-24-23843711-3923; fax: 86-24-23896576