金针菇中具抑瘤活性的碱性蛋白火菇素的分离纯化和性质研究

金针菇中具抑瘤活性的碱性蛋白火菇素的分离纯化和性质研究

陈畅, 薛久刚, 周凯松, 李彦, 张晗星, 张长铠^*

山东大学微生物技术国家重点实验室, 济南 250100 中国

收稿日期:2003-02-12 修回日期:2003-05-10 出版日期:2003-06-15 发布日期:2003-06-15

Purification and Characterization of Flammulin,a Basic Protein with Anti-tumor Activities from Flammulina velutipes

CHEN Chang, XUE Jiu-gang, ZHOU Kai-song, LI Yan, ZHANG Han-xing, ZHANG Chang-kai^*

State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China

Received:2003-02-12 Revised:2003-05-10 Online:2003-06-15 Published:2003-06-15

摘要/Abstract

摘要： 目的对一种具有抑瘤活性的碱性蛋白火菇素进行分离纯化和性质研究。方法应用硫酸铵、乙醇分级沉淀,柱色谱等方法进行分离纯化。电泳分析、氨基酸组成分析、质谱分析等方法被用于火菇素的理化性质研究。结果火菇素被提纯至电泳纯并结晶。经性质研究确定火菇素的分子量为19891.13 Da, pI = 8.9, λmax = 276～278 nm, λmin = 250nm, 并缺少甲硫氨酸。通过基质辅助激光解吸离子化质谱(MALDI-MS)分析, 测得了火菇素的指纹图谱, 表明它为一种新蛋白。结论火菇素被首次提纯至电泳纯, 并首次叙述了它的理化性质。

关键词: 抑瘤活性, 火菇素, 金针菇, 纯化, 性质研究, 质谱

Abstract: Aim To purify and characterize flammulin, a basic protein with anti-tumor activities. Methods Ammonium sulfate, ethanol fractionation and column chromatography were used for separation and purification. Electrophoretic analysis, amino acid analysis, and MS of flammulin were carried out. Results Flammulin was purified to electrophoretic homogeneity and crystallized. With a molecular mass of 19891.13 Da, pI = 8.9, λmax = 276-278 nm, λmin = 250nm, flammulin was characterized by its lack of methionine. Fingerprint mapping of flammulin was determined by MALDI-MS following in-gel protease digestion; no close matches were identified. Conclusion Flammulin was purified to electrophoretic neity, and its characteristics are discussed for the first time.

Key words: anti tumor activities, anti tumor activities, flammulin , flammulin , Flammulina velutipes, Flammulina velutipes, purification , purification , characterization , characterization , massspectrometry, massspectrometry

中图分类号:

Q936

Q946

Supporting: ^*Corresponding author. Tel.: 86-531-8364427

陈畅, 薛久刚, 周凯松, 李彦, 张晗星, 张长铠^*. 金针菇中具抑瘤活性的碱性蛋白火菇素的分离纯化和性质研究[J]. .

CHEN Chang, XUE Jiu-gang, ZHOU Kai-song, LI Yan, ZHANG Han-xing, ZHANG Chang-kai^*. Purification and Characterization of Flammulin,a Basic Protein with Anti-tumor Activities from Flammulina velutipes[J]. .

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