关键词: 精油, 水煎液, 血清, 高效液相色谱

Abstract:

In the present study, we aimed to determine the major components responsible for the analgesic effects of Dahurian Angelica root. The rats were administered with either the volatile oil or decoction of Dahurian Angelica root. Formalin plantar injection test was employed as the pain model. The rats were observed for nociception, after which their blood was collected fromthe abdominal aorta. High-performance liquid chromatography (HPLC) fingerprints of serum were performed. The results demonstrated that no remarkable analgesic effect was observed from the low-dose decoction group, whereas the other doses showed pain-relieving effects in the rat model. The components of the rat sera in the different treatments were determined by HPLC. Common chromatographicpeaks were observed at 24.941, 25.755, 29.854, 32.761 and 33.928 min, representing the major constituents of the decoction and volatile oil of Dahurian Angelica root. The analgesic components of Dahurian Angelica root obtained from the rat serum mainly peaked at 24.941, 25.755, 29.854, 32.761 and 33.928 min, suggesting trace components and metabolites.

Key words: Volatile oil, Decoction, Serum, HPLC chromatogram