RP-HPLC-UV法同时测定萹蓄中8个黄酮类化合物的含量

doi:10.5246/jcps.2014.03.022

中国药学(英文版)

RP-HPLC-UV法同时测定萹蓄中8个黄酮类化合物的含量

付庆荣, 刘淑娟, 王弘, 陈世忠^*

北京大学医学部药学院中药研究室, 北京 100191

收稿日期:2013-07-24 修回日期:2013-10-13 出版日期:2014-03-13 发布日期:2013-10-28
通讯作者: *Corresponding author. Tel.: 86-10-82802723; Fax: 86-10-82802723; E-mail: chenshizhong66@163.com
作者简介:*Corresponding author. Tel.: 86-10-82802723; Fax: 86-10-82802723; E-mail: chenshizhong66@163.com
基金资助:
Study of Nature of Geo-authentic Crude Drug (“973” State Key Project, Grant No. 2006CB504700).

Simultaneous determination of eight flavonoids in Polygonum aviculare L. by RP-HPLC-UV

Qingrong Fu, Shujuan Liu, Hong Wang, Shizhong Chen^*

Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2013-07-24 Revised:2013-10-13 Online:2014-03-13 Published:2013-10-28
Contact: *Corresponding author. Tel.: 86-10-82802723; Fax: 86-10-82802723; E-mail: chenshizhong66@163.com
About author:*Corresponding author. Tel.: 86-10-82802723; Fax: 86-10-82802723; E-mail: chenshizhong66@163.com
Supported by:
Study of Nature of Geo-authentic Crude Drug (“973” State Key Project, Grant No. 2006CB504700).

摘要/Abstract

摘要：

建立了反相高效液相色谱法同时测定扁蓄药材中8个黄酮 (杨梅苷, 金丝桃苷, 木犀草苷, 扁蓄苷, 槲皮苷, 槲皮素, 木犀草素和山奈酚)的含量。采用Inertsil ODS-4 色谱柱, 乙腈–0.5%磷酸水溶液梯度洗脱, 流速为1 mL/min, 柱温为35 ºC,检测波长为360 nm。上述8个黄酮类成分达到基线分离, 标准曲线线性关系良好 (r²>0.9991), 日内和日间精密度、稳定性和重复性实验均符合要求, 加样回收率为95.58%–102.65%。对28批不同产地的扁蓄样品进行了含量测定和聚类分析,结果表明该方法不仅可以对萹蓄药材进行质量控制, 对于萹蓄药材的道地性分析也提供了科学数据。

关键词: 萹蓄, 黄酮, 定量分析, 聚类分析

Abstract:

A reliable high performance liquid chromatography method was developed for the quality evaluation of Polygonum aviculare L. Eight marker flavonoids were identified and simultaneously quantified, which included myricitrin, hyperoside, galuteolin, avicularin, quercitrin, quercetin, luteolin, and kaempferol. The analysis was performed on an Inertsil ODS-4 column (4.6 mm×150 mm, 5 µm) with gradient elution. The mobile phases were 0.5% aqueous phosphoric acid and acetonitrile. The detection wavelength was 360 nm. The eight marker flavonoids were separated well with good linearity (r²>0.9991), precision, stability and repeatability. The recovery rate was 95.58%–102.65%. Cluster analysis was employed to analyze 28 batches of samples. The result indicated that this method provides an efficient way to perform quality control as well as a scientific rationale for the Geo-authentication of Polygonum aviculare L.

Key words: Polygonum aviculare L., Flavonoids, Quantitative analyses, Cluster analysis

中图分类号:

R284.1

Supporting: Study of Nature of Geo-authentic Crude Drug (“973” State Key Project, Grant No. 2006CB504700).

付庆荣, 刘淑娟, 王弘, 陈世忠. RP-HPLC-UV法同时测定萹蓄中8个黄酮类化合物的含量[J]. 中国药学(英文版), DOI: 10.5246/jcps.2014.03.022.

Qingrong Fu, Shujuan Liu, Hong Wang, Shizhong Chen. Simultaneous determination of eight flavonoids in Polygonum aviculare L. by RP-HPLC-UV[J]. Journal of Chinese Pharmaceutical Sciences, DOI: 10.5246/jcps.2014.03.022.

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RP-HPLC-UV法同时测定萹蓄中8个黄酮类化合物的含量

Simultaneous determination of eight flavonoids in Polygonum aviculare L. by RP-HPLC-UV

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