线粒体是双乙酰丙酮氧钒在肾上皮细胞中诱导产生活性氧物种的主要来源

doi:10.5246/jcps.2013.01.010

线粒体是双乙酰丙酮氧钒在肾上皮细胞中诱导产生活性氧物种的主要来源

侯聪聪, 王刚, 杨晓改^*

北京大学医学部药学院化学生物学系, 北京 100191

收稿日期:2012-08-20 修回日期:2012-12-01 出版日期:2013-01-20 发布日期:2013-01-20
通讯作者: 杨晓改*

Mitochondria as the main source of vanadyl acetylacetonate-induced reactive oxygen species generation in renal epithelial cell lines

Congcong Hou, Gang Wang, Xiaogai Yang^*

Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2012-08-20 Revised:2012-12-01 Online:2013-01-20 Published:2013-01-20
Contact: Xiaogai Yang*

摘要/Abstract

摘要： 本研究利用具有降糖效应的双乙酰丙酮氧钒确定其在两种肾上皮细胞系LLC-PK₁和MDCK中诱导产生活性氧物种的来源。利用四种常用的荧光试剂分别检测了VO(acac)₂诱导产生的过氧化氢(H₂O₂)和超氧阴离子(·O₂^-)的水平并确定了它们在细胞内的主要来源部位。实验结果表明, VO(acac)₂在LLC-PK₁和MDCK两种肾细胞系中均能显著诱导ROS的生成, 并且ROS主要来源于线粒体。本研究结果提示, 可通过局部降低线粒体部位ROS的水平来减少钒化合物的毒性损伤, 同时不影响钒化合物的活性。

关键词: VO(acac)2, 线粒体, 活性氧物种, LLC-PK1细胞, MDCK细胞

Abstract:

In this study, we aimed to clarify the source of the reactive oxygen species (ROS) generation induced by vanadium compounds. We used vanadyl acetylacetonate (VO(acac)₂), a highly effective agent in controlling hyperglycemia, to determine the source of ROS generation in two renal cell lines LLC-PK₁ and MDCK. Four commonly fluorescent dyes were used to assess VO(acac)₂-induced H₂O₂ and ·O₂^- production and their location. It demonstrated that VO(acac)₂can induce significant ROS generation in both LLC-PK₁ and MDCK cells, which were primarily derived from mitochondria. The results obtained in this study raised the possibility to reduce ROS level induced by vanadium compounds locally and thus avoid affecting its activity.

Key words: VO(acac)2, Mitochondria, Reactive oxygen species, LLC-PK1 cells, MDCK cells

中图分类号:

R915

Supporting:

Foundation items: National Natural Science Foundation of China (Grant No. 20871008 and 21171011).
^*Corresponding author. Tel.: 86-10-82801539

侯聪聪, 王刚, 杨晓改^*. 线粒体是双乙酰丙酮氧钒在肾上皮细胞中诱导产生活性氧物种的主要来源[J]. , DOI: 10.5246/jcps.2013.01.010.

Congcong Hou, Gang Wang, Xiaogai Yang^*. Mitochondria as the main source of vanadyl acetylacetonate-induced reactive oxygen species generation in renal epithelial cell lines[J]. , DOI: 10.5246/jcps.2013.01.010.

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