Antihyperglycemic, insulin-sensitizing and antioxidant activities of the active fractions from Anoectochilus chapaensis

doi:10.5246/jcps.2014.04.037

Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (4): 262-267.DOI: 10.5246/jcps.2014.04.037

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Antihyperglycemic, insulin-sensitizing and antioxidant activities of the active fractions from Anoectochilus chapaensis

Jinyan Cai^*, Lin Zhao, En Zhu, Chengxi Wang

1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China
2. School of Life Science and Bio-pharmaceutical, Guangdong Pharmaceutical University, Guangzhou 510006, China

Received:2013-12-18 Revised:2014-02-22 Online:2014-04-28 Published:2014-02-27
Contact: *Corresponding author. Tel.: +86-15920107845; Fax: +86-20-39352129; E-mail: caijy928@163.com
About author:*Corresponding author. Tel.: +86-15920107845; Fax: +86-20-39352129; E-mail: caijy928@163.com
Supported by:
National Natural Science Foundation of China (Grant No. 81001628) and Guangdong Natural Science Foundation (Grant No. S2013010014771).

Abstract

Abstract:

Anoectochilus chapaensisGagnep. (Orchidaceae), an indigenous and valuable Chinese folk medicine, has been widely used in China to treat diabetes. However, few reports are available about its constituents and activity. The present experiment was conducted to investigate the active fractions from A. chapaensis in diabetic rat model induced by high-fat diet plus streptozotocin. The total EtOH extract from the whole herbs of A. chapaensis,half of which was partitioned in sequence with petrol ether (PE), ethyl acetate (EtOAc), n-BuOH and H₂O, thus yielding four fractions, all of them were orally administered with an identical dose amount to 4 g/kg dried crude herbs once a day for consecutive two weeks to further investigate the antihyperglycemic activity. The EtOAc fraction caused a significant fall in the non-fasting blood glucose level of diabetic rats from (402.66±82.26) to (226.26±62.10) mg/dl, which may be attributed to ameliorating insulin resistance, modulating the activity of enzymatic antioxidants, reducing the content of NO, etc. Much more intact β cells in the islets of Langerhans in EtOAc fraction-treated groups than the negative control were observed, which greatly supported the morphological and functional elucidation. The OGTT evidencedthat EtOAc fraction could promote the endurance capacity of acute glucose increase in diabetic rats. The EtOAc fraction ofA. chapaensis contains some hypoglycemic and antioxidant principles with the potential to be developed further for the treatment of diabetes specifically associated with an insulin resistance state.

Key words: Anoectochilus chapaensis, Antihyperglycemic, ISI, Active fraction

CLC Number:

R962

Supporting:

Jinyan Cai, Lin Zhao, En Zhu, Chengxi Wang. Antihyperglycemic, insulin-sensitizing and antioxidant activities of the active fractions from Anoectochilus chapaensis[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(4): 262-267.

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Antihyperglycemic, insulin-sensitizing and antioxidant activities of the active fractions from Anoectochilus chapaensis

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