The aim of this study is to isolate and identify the chemical compounds in ethanol extract of Cyclocarya paliurus. Some purification and analysis techniques like silica gel, D101-macroporous adsorptive resins, and Sephadex LH-20 column chromatographies as well as high-performance liquid chromatography were used to isolate and analyze the compounds from ethanol extract of Cyclocarya paliurus. The structures of these constituents were identified by spectroscopic techniques such as nuclear magnetic resonance and high-resolution mass spectrometries. Twenty-eight compounds, including flavonoids and their glycoside, carbohydrate, coumarin and organic acid, were isolated from ethyl acetate and n-butanol fractions in ethanol extract of Cyclocarya paliurus, and they were identified as kaempferol (1), coumestrol (2), kaempferol 3-O-β-D-glucoside (3), methyl caffeoylquinic acid (4), coptichic aldehyde (5), schizandriside (6), kaempferol 3-O-α-L-rhamnoside (7), 3-caffeoylquinic acid ethyl ester (8), quercetin (9), luteolin (10), protocatechuic acid (11), kaempferol-3-O-α-L-furan arabinose (12), trans-p-hydroxy-cinnamic acid (13), α-D-glucopyranosido-β-D-fructofuranoside, sucrose (14), peucedanol (15), chlorogenic acid (16), pyridoxine (17), quercetin-3-O-β-D-glucuronide (18), kaempferol-3-O-β-D-glucuronide (19), isoquercitrin (20), mururin A (21), citroside A (22), benzyl-6-O-α-L-arabinofuranosyl-β-D-glucopyranoside (23), (+)-(6S,9R)-9-O-β-D-glucopyranosyl-6-hydroxy-3-O-α-ionol (24), myricetin-3-O-β-D-glucopyranoside (25), (4R)-4-(3-Oxo-1-buten-1-ylidene)-3α,5,5-trimethylcyclohexane-1α,3β-diol (26), quercetin-3-O-α-L-rhamnopyranosyl (27) and 3,5-O-dicaffeoylquinic acid (28). Compounds 2, 5, 6, 15, 21, 22, 23, 24, 26 and 28 were isolated from Cyclocarya paliurus for the first time.
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