Optimization of ultrasound assisted extraction of celastrol from Celastrus monospermus Roxb. using response surface methodology

doi:10.5246/jcps.2017.02.012

Abstract

Abstract:

In the present study, we aimed to investigate the effects of key extraction parameters including extraction time (10-20 min),extraction temperature (30-60 °C), ultrasonic power (60-90 W) and solvent-to-solid (S/S) ratio (10-30 mL/g) on yield of celastrol from Celastrus monospermus Roxb. To optimize the conditions, we investigated the effects of parameters on the ultrasound assisted extraction (UAE) with the Box-Behnken Design (BBD), one widely used form of Response Surface Methodology (RSM). In all tested solvents, ethanol was the most effective for celastrol extraction, followed by methanol, ethanol, ethyl acetate, n-butanol and water. A second order polynomial model was fitted well to the extraction experimental data with R² of 0.9928. Extraction yield of 3.116 mg/g was obtained for celastrol under the optimized extraction conditions of extraction time (20 min), extraction temperature (46 °C), ultrasonic power (60 W) and S/S ratio (30 mL/g). Experimental validation was performed, and the experimental values agreed well with the predicted values. The results indicated that the UAE was good extraction material for celastrol from C. monospermus Roxb.

Key words: Celastrol, Box-behnken design, Response surface methodology, Ultrasound assisted extraction, Analysis

CLC Number:

R284

Supporting:

Chaohai Pang, Gong Fang, Dingyong Wang. Optimization of ultrasound assisted extraction of celastrol from Celastrus monospermus Roxb. using response surface methodology[J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(2): 130-138.

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