Evaluation of the stability of droplets in emulsion using multisample analytical centrifugation

doi:10.5246/jcps.2023.07.048

Abstract

Abstract:

In the present study, we aimed to evaluate the stability of droplets in the emulsion during storage and distinguish the stability of different prescriptions. The multisample analytical centrifugation-LUMisizer was applied to evaluate the impact of freeze-thaw cycles on droplets, combined with droplet size parameters, zeta potential, and observation by cryo-transmission electronic microscopy. After freeze-thaw cycles, the droplets showed significant changes in characters, globule size distribution, and light transmission. After freeze-thaw cycles, the droplets were demulsified (oil droplets and creaming were observed). Formulations with different contents of propofol (10:1 and 20:1, m/v) showed significant differences in the behavior of the droplets. Droplets by accelerated destruction could be distinguished by LUMisizer, which could quickly identify the stability and effectively supplement the traditional methods, offering better quality control of droplets in the emulsion.

Key words: Droplet particle, Emulsion, Stability analysis, Multisample analytical centrifugation, Light transmission, Freeze-thaw cycle, Oil-in-water

Supporting:

Chunyan Xie, Feng Yue, Haoquan Yu, Canzhong Huang, Shaoqun Zeng. Evaluation of the stability of droplets in emulsion using multisample analytical centrifugation[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(7): 574-586.

Figures/Tables 13

Table 1. Particle size parameters and zeta potential.

Table 2. Stability index.

Table 3. Instability indexes and particle size parameters.

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