Simultaneous determination of ginsenosides Rg1, Re and Rb1 in rat plasma by HPLC and its application to pharmacokinetic study of SHENMAI injection

doi:10.5246/jcps.2015.07.061

Abstract

Abstract:

In the present study, we developed a sensitive and efficient high performance liquid chromatography (HPLC) method for the simultaneous determination of three ginsenosides (Rg₁, Re, Rb₁) in rat plasma. Chromatographic separation was performed on a C₁₈ (150 mm×4.6 mm) column utilizing gradient elution profile and a mobile phase consisting of (A) water and (B) acetonitrile. The calibration curve, with a great correlation coefficient greater than 0.998, was linear over the range of 1.0–30.0 μg/mL for ginsenoside Rg₁, 0.5–15.0 μg/mL for ginsenoside Re, and 0.5–200.0 μg/mL for ginsenoside Rb₁. The intra- and inter-day precisions for three ginsenosides (Rg₁, Re, Rb₁) were all less than 6.0%, and average recovery, examined at three concentration levels, ranged from 96.1% to 118.6%. The samples was stable within 24 h at 4 ºC storage, and 30 d at –20 ºC storage with three freeze-thaw-assay cycles. The low limits of quantification (LOQ) were 1.0, 0.5 and 0.5 μg/mL for Rg₁,Re and Rb₁, respectively. Taken together, the newly developed method was successfully applied to study the pharmacokinetics of ginsenoside Rg₁, Re and Rb₁ in rat plasma after intravenous administration of SHENMAI injection (SMI).

Key words: Ginsenoside Rg1, Re, Rb1, HPLC, SHENMAI injection, Plasma

CLC Number:

R969.1

Supporting:

Qingdan Xue, Peng Wang, Yuhong Kang, Qiuhong Li. Simultaneous determination of ginsenosides Rg1, Re and Rb1 in rat plasma by HPLC and its application to pharmacokinetic study of SHENMAI injection[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(7): 475-481.

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