Preparation optimiztion of compound Codonopsis Radix granules and simultaneous determination of its four components

doi:10.5246/jcps.2023.08.055

Abstract

Abstract:

In the present study a single-factor experiment and response surface methodology were utilized to optimize the spray-drying process of compound Codonopsis Radix granules and determine the optimal spray-drying process parameters. The proportions of excipients were examined using indicators of molding rate and moisture absorption rate. High-performance liquid chromatography (HPLC) was established to simultaneously determine the contents of lobetyolin, ferulic acid, calycosin-7-O-β-D-glucoside (CG), and glycyrrhizin in the compound Codonopsis Radix granules. The findings indicated that the highest spray-drying yield was achieved when the inlet temperature of spray drying was set at 145 oC, the inlet air velocity at 75%, the feed rate at 8%, and the drying aid at 5%. A ratio of paste starch to powdered sugar at 2:1 resulted in a good particle molding rate and moisture absorption rate. By effectively optimizing the spray-drying process of compound Codonopsis Radix granules and excipient dosage this study provides a valuable reference for the production and preparation of these granules. The contents of lobetyolin, ferulic acid, glycyrrhizin, and CG in the compound Codonopsis Radix granules were found to be 0.38, 0.22, 0.45, and 0.25 mg/g, respectively. These results provided a foundation for establishing a quality control method for compound Codonopsis Radix granules.

Key words: Compound Codonopsis Radix granules, Response surface optimization, Spray drying, High-performance liquid chromatography

Supporting:

Jie Yang, Caifang Jing, Shaochuang Li, Sitong Liu, Dongming Li, Qinqing Li, Wenbin He. Preparation optimiztion of compound Codonopsis Radix granules and simultaneous determination of its four components[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(8): 665-676.

Figures/Tables 7

Table 1. Factor levels of spray drying experiments.

Table 2. Design and results of compound Codonopsis Radix granules spray drying response surface test (n = 3).

Table 3. Screening results of excipient ratio (n = 3).

Table 4. Results of methodological investigation (n = 6).

Table 5. Test results of sample recovery rate of each component (n = 6).

Table 6. Determination results of each component content (mg/g, n = 6).

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