The in vitro antioxidant activities of the ethanol extract and its different polar fractions from Ephedra saxatilis Royle ex Florin in Tibet

doi:10.5246/jcps.2023.06.041

Abstract

Abstract:

In the present study, we aimed to investigate the in vitro antioxidant activity of the ethanol extract and its polar extracts from Ephedra saxatilis Royle ex Florin in Tibet. The chemical constituents were first screened by the preliminary phytochemical methods. The contents of total polyphenols and total flavonoids were identified by the Folin-Ciocalteu method and colorimetric method of the aluminum chloride method, respectively. Furthermore, their antioxidant activities were investigated by the DPPH method, ABTS method, hydroxyl radical scavenging, and reduction powder method. The preliminary phytochemical screening showed that the polyphenols, glycosides, flavonoids, and saponins existed in ethanol extract, and the chemical composition in different polar extracts varied, among which the contents of polyphenols and flavonoids were highest in the ethyl acetate part, followed by the dichloromethane part, exhibiting best antioxidant capacity. Both the ethyl acetate and dichloromethane parts from Ephedra saxatilis Royle ex Florin exhibited better antioxidant activities than BHT, which was associated with the high contents of polyphenols and flavonoids.

Key words: Ephedra saxatilis Royle ex Florin, Ethanol extract, Different polar parts, Antioxidants, Constituents

Supporting:

Han Wang, Fanglong Li, Aga Er-bu, Xiaoxia Liang, Geng Sang, Car Rangnanjia. The in vitro antioxidant activities of the ethanol extract and its different polar fractions from Ephedra saxatilis Royle ex Florin in Tibet[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(6): 478-486.

Figures/Tables 6

Table 1. The consequence of preliminary phytochemical screening.

Table 2. The consequence of polyphenol content determination.

Table 3. The consequence of flavonoid content determination.

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