Abstract:

Phytochemical investigations of the aerial parts of Reineckia carnea, collected in Yunnan Province of China, were conducted to explore new chemical constituents. A series of chromatographic and spectroscopic procedures were utilized on the aqueous solution after partitioned with ethyl acetate, which resulted in the separation of a new furostanol-type glycoside and four known compounds. The structures of the isolated compounds were elucidated on the basis of spectroscopic techniques (1D and 2D NMR, IR, HRESIMS) as 26-O-β-D-glucopyranosyl-(25S)-5β-furost-20(22)-en-1α,3β,26-triol-1-O-α-L-arabinopyranosyl-(1→2)-[α-L-rhamnopyranos-yl]-3-O-α-L-rhamnopyranoside (1), (1β,3β,16β,22S)-cholest-5-en-1,3,16,22-tetrol-1,16-di-(β-D-glucopyranoside) (2), diosgenin (3), β-sitosterol (4), ecdysterone (5).

Key words: Reineckia carnea, Steroidal glycosides, Steroids, Structural identification