Abstract:

In the present research, in order to study the chemical constituents of Paliurus ramosissimus (Lour.) Poir., the isolation of its ingredients was performed by repeated chromatography on silica gel, Sephadex LH-20, ODS and preparative HPLC. Their structures were elucidated on the basis of combination of mass spectrometry and 1D, 2D NMR spectroscopy. A total of 8 compounds were obtained, and their structures were identified as 3β-hydroxy-27-(3,4-dihydroxybenzoyl)oxylup-20(29)-en-28-oic acid(1),betulinic acid (2), lupeol(3), 27-O-trans-caffeoylcylicodiscic acid (4), ceanothic acid (5), 24-hydroxyceanothic acid (6), dihydrokaempferol (7), eriodictyol (8). Among them, compound 1 was a new compound. Compounds 3–4, 7–8 were isolated from Paliurus ramosissimus (Lour.) Poir. for the first time.

Key words: Paliurus ramosissimus, Chemical constituents, Structural identification