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柱分配色谱-毛细管区带电泳联用分析板蓝根中五种微量有机酸

柴逸峰*, 纪松岗, 张国庆, 刘长海   

  1. 1.第二军医大学药学院, 上海 200433;
    2.解放军401医院, 青岛 266071;
    3.东方肝胆外科医院, 上海 200433;
  • 收稿日期:2003-06-20 修回日期:2003-11-10 出版日期:2003-12-15 发布日期:2003-12-15
  • 通讯作者: 柴逸峰*

Determination of Five Organic Acids in Radix Isatidis by Column Partition Chromatography and Capillary Zone Electrophoresis

CHAI Yi-feng*, JI Song-gang, ZHANG Guo-qing, LIU Chang-hai   

  1. 1.School of Pharmacy, Second Military Medical University, Shanghai 200433, China;
    2.Department of Pharmacy, Eastern Institute and Hospital of Hepatobiliary Surgery, Shanghai 200438, China;
    3.The 401st Hospital of PLA, Qingdao 266071, China
  • Received:2003-06-20 Revised:2003-11-10 Online:2003-12-15 Published:2003-12-15
  • Contact: CHAI Yi-feng*

摘要: 目的 测定板蓝根中五种微量有机酸水杨酸, 苯甲酸, 丁香酸, 邻氨基苯甲酸和喹唑酮酸进行分离和含量测定. 方法考察了板蓝根的提取方法, 建立了柱分配色谱-毛细管区带电泳联用分析五种微量有机酸的方法. 结果 水杨酸, 苯甲酸, 丁香酸, 邻氨基苯甲酸和喹唑酮酸的线性范围分别5.52-92.0 μg·mL-1, 5.12-102 μg·mL-1, 2.28-84.4 μg·mL-1, 4.78-159 μg·mL-1 and 1.74-87.0 μg·mL-1. 结论 该方法快速、简便、准确、可靠.

关键词: 板蓝根, 有机酸, 毛细管电泳, 柱分配色谱

Abstract: Aim To determine five organic acids in Radix Isatidis. Method The extraction method and the column partition chromatographic conditions were studied. Then a capillary zone electrophoretic method was set up for the determina-tion. Results The linear ranges of quinazolinone acid, n-anthranilic acid, benzoic acid, salicylic acid, and syringic acid were 5.52-92.0 μg·mL-1, 5.12-102 μg·mL-1, 2.28-84.4 μg·mL-1, 4.78-159 μg·mL-1, and 1.74-87.0 μg·mL-1 respectively. Conclusion The established method is accurate and simple.

Key words: Radix Isatidis, Radix Isatidis, organic acids, organic acids, capillary electrophoresis, capillary electrophoresis, column partition chromatography, column partition chromatography

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Supporting: Foundation item: Science and Technology Development Foundation of Shanghai under Grant No. 01DJ19012.
*Corresponding author. Tel.: 021-25070373