罗汉果皂苷IVa碳氢NMR信号的归属

罗汉果皂苷IVa碳氢NMR信号的归属

张建业, 杨秀伟^*

1.北京大学天然药物及仿生药物国家重点实验室;
2.北京大学医药卫生分析中心, 北京 100083

收稿日期:2003-03-16 修回日期:2003-11-10 出版日期:2003-12-15 发布日期:2003-12-15
通讯作者: 杨秀伟*

Assignments of ¹H and ¹³C NMR Signals of Mogroside IVa

ZHANG Jian-ye, YANG Xiu-wei^*

1.State Key Laboratory of Natural and Biomimetic Drugs, Peking University;
2.Medical and Healthy Analysis Center, Peking University, Beijing 100083, China

Received:2003-03-16 Revised:2003-11-10 Online:2003-12-15 Published:2003-12-15
Contact: YANG Xiu-wei*

摘要/Abstract

摘要： 目的研究传统中药罗汉果的皂苷类化学成分罗汉果皂苷Ⅳa的结构,总结其结构的NMR特点. 方法采用常法提取、分离、纯化罗汉果皂苷IVa, 应用NMR方法(¹H NMR、¹³C NMR、DEPT、¹H-¹H COSY、HSQC、HMBC和NOESY)和计算机分子模拟研究其结构. 结果对罗汉果皂苷IVa的¹H和¹³C信号进行了归属, 并为该类型化合物的结构确定提供了波谱学依据. 结论包括¹H-¹H COSY、HSQC、HMBC、NOESY的一维和二维核磁共振波谱技术是对结构进行无创伤性分析的有力工具.NMR方法确定的结构与计算机分子模拟的最优构象一致.

关键词: 罗汉果, 罗汉果皂苷IVa, 三萜皂苷, 核磁共振波谱, 归属

Abstract: Aim To investigate the structure of mogroside Iva isolated from traditional Chinese medicine fructus mo-mordicae [fruits of Siraitia grosvenori (Swingle) C. Jeffery] and summarize the NMR characteristics of the structure. Methods Common extraction, separation and purification methods were used. Various NMR techniques including ¹H NMR,¹³C NMR, DEPT, ¹H-¹H COSY, HSQC, HMBC, NOESY and molecular model simulated by computer were used to elucidatethe structure. Results ¹H and ¹³C NMR signals of mogroside IVa were assigned, and spectroscopic basis was obtained for identification of such type of compounds. Conclusion 1D and 2D NMR techniques including ¹H-¹H COSY, HSQC, HM-BC, NOESY spectra are powerful tools for structure analysis. The structure determined by NMR methods is identical with energy minimized conformation simulated by computer.

Key words: fructus momordicae, fructus momordicae, mogroside IVa, mogroside IVa, triterpenoid saponin, triterpenoid saponin, NMR spectrum, NMR spectrum, assignment, assignment

中图分类号:

R284.1

R917.02

Supporting: ^*Corresponding author. Tel.: 010-62070317; 010-82801569

张建业, 杨秀伟^*. 罗汉果皂苷IVa碳氢NMR信号的归属[J]. .

ZHANG Jian-ye, YANG Xiu-wei^*. Assignments of ¹H and ¹³C NMR Signals of Mogroside IVa[J]. .

[1]	刘晓婷, 徐静娜, 肖坤, 郭强胜, 许旭. 固相萃取-定量核磁共振波谱法测定双黄连胶囊中的绿原酸[J]. 中国药学(英文版), 2020, 29(4): 227-235.
[2]	吴琼, 涂光忠, 付宏征. 九子参中一个新三萜皂苷[J]. 中国药学(英文版), 2014, 23(4): 246-250.
[3]	周渊, 李宁, 张加余, 姜勇, 屠鹏飞. RP-HPLC-DAD法同时测定毛冬青根中4种三萜皂苷和2种木脂素的含量[J]. 中国药学(英文版), 2014, 23(12): 866-872.
[4]	王超, 李军, 屠鹏飞. 岗梅叶的化学成分研究[J]. 中国药学(英文版), 2014, 23(11): 778-782.
[5]	宋丽军, 谭晓梅, 赵文昌, 罗佳波^*. HPLC-DAD法同时定量分析葛根芩连微丸中12种活性成分[J]. , 2010, 19(6): 464-470.
[6]	王刊, 付宏征^*. 肥皂荚Gymnocladus chinensis Baill.中两个三萜皂苷的结构鉴定[J]. , 2009, 18(2): 141-145.
[7]	郭杰, 杨秀伟^*. 浙江七叶树种子中三萜皂苷成分的研究[J]. , 2004, 13(2): 87-91.
[8]	杨春晖, 李勤, 刘雪辉, 赵兴凯, 崔育新^*. 氨氯地平和利培酮的¹H和¹³C核磁共振信号归属[J]. , 2004, 13(1): 49-52.