反向毛细管电泳法测定不同产地远志中细叶远志皂苷的含量

doi:10.5246/jcps.2011.04.042

反向毛细管电泳法测定不同产地远志中细叶远志皂苷的含量

郝增燕, 王洁, 张一鸣, 陈安家^*

山西医科大学药学院, 山西太原 030001

收稿日期:2011-01-21 修回日期:2011-05-10 出版日期:2011-07-01 发布日期:2011-07-01
通讯作者: 陈安家*

Determination of tenuifolin in Polygalae Radix from different regions by RF-HPCE

Zeng-Yan Hao, Jie Wang, Yi-Ming Zhang, An-Jia Chen^*

College of Pharmacy, Shanxi Medical University, Taiyuan 030001, China

Received:2011-01-21 Revised:2011-05-10 Online:2011-07-01 Published:2011-07-01
Contact: An-Jia Chen*

摘要/Abstract

摘要： 首次建立了简单、经济、有效的反向毛细管电泳分离测定远志药材中细叶远志皂苷含量的方法。通过对分离条件的选择优化, 采用含有35%甲醇(v/v) 的50 mmol/L的硼砂缓冲液使待测成分得到完全分离; 细叶远志皂苷在0.0563-0.9000 mg/mL浓度范围内呈良好线性关系, r = 0.9988, 加样回收率在99.50%-104.7%之间。结果表明该方法简便、快速、准确可靠, 可作为远志的质量控制方法。

关键词: 反向毛细管电泳, 细叶远志皂苷, 远志

Abstract: A simple, low-cost and effective reverse flow-high performance capillary electrophoresis (RF-HPCE) method was developed for the separation and determination of tenuifolin in Polygalae Radix. Through optimization of separation conditions, a buffer of 50 mmol/Lborax containing methanol (65:35, v/v) was selected for the separation of analytes. Regression equation revealed good linear relationship between peak area and concentration of the analyte in the range of 0.0563-0.9000 mg/mL (r = 0.9988). The recoveries ranged from 99.50%-104.7%. The results indicated that this method is simple, rapid and accurate. It can be used for the quality control of Polygalae Radix.

Key words: RF-HPCE, Tenuifolin, Polygalae Radix

中图分类号:

R284.2

Supporting:

Foundation item: Natural Science Foundation of Shanxi Province (Grant No. 200801107-1).

^*Corresponding author. Tel.: 15835108120;
E-mail: chenanjia888@163.com

郝增燕, 王洁, 张一鸣, 陈安家^*. 反向毛细管电泳法测定不同产地远志中细叶远志皂苷的含量[J]. , DOI: 10.5246/jcps.2011.04.042.

Zeng-Yan Hao, Jie Wang, Yi-Ming Zhang, An-Jia Chen^*. Determination of tenuifolin in Polygalae Radix from different regions by RF-HPCE[J]. , DOI: 10.5246/jcps.2011.04.042.

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[3]	周雨虹, 姜勇, 温晶, 陈玉平, 屠鹏飞*. 卵叶远志根的化学成分研究 [J]. , 2008, 17(2): 148-152.