高效液相色谱法对中药复方四君子汤中异甘草素、甘草素、异甘草苷和甘草苷的含量测定

高效液相色谱法对中药复方四君子汤中异甘草素、甘草素、异甘草苷和甘草苷的含量测定

刘扬, 杨峻山^*

中国协和医科大学中国医学科学院药用植物研究所, 北京 100094

收稿日期:2005-06-27 修回日期:2005-11-10 出版日期:2005-12-15 发布日期:2005-12-15
通讯作者: 杨峻山*

Determination of Liquiritigenin, Liquiritin, Isoliquiritigenin and Isoliquiritin in Extract of Traditional Chinese Medicine Sijunzi Decoction by High-Performance Liquid Chromatography

LIU Yang, YANG Jun-shan^*

Institute of Medicinal Plant Development, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100094, China

Received:2005-06-27 Revised:2005-11-10 Online:2005-12-15 Published:2005-12-15
Contact: YANG Jun-shan*

摘要/Abstract

摘要： 目的为中药复方四君子汤建立简便的液相分析方法.对复方提取物中异甘草素、甘草素、异甘草苷和甘草苷进行定量分析。方法色谱条件为(A) 0.04%甲酸溶液(pH 3)和(B)乙腈; 0 - 5 min 10% B, 45 min达到90% B; 在室温下C18反相柱, 流速为0.2 mL·min^-1实现分离和含量测定; 检测波长为280 nm和368 nm。结果四君子汤提取物中(25 mg·mL^-1)含有1.47 μg·mL^-1甘草素, 16.40 μg·mL^-1甘草苷, 0.66 μg·mL^-1异甘草素, 2.12 μg·mL^-1异甘草苷; 回收率分别为102.2%, 97.7%, 100.3%, 和 99.9%。结论本文首次报道复方四君子汤中多个黄酮类成分的含量测定。

关键词: 中药, 中药, 中药, 四君子汤, 四君子汤, 四君子汤, 高效液相色谱法, 高效液相色谱法, 高效液相色谱法

Abstract: Aim An HPLC-UV method for the analysis of Traditional Chinese Medicine (TCM) preparation, Sijunzi decoction, has been developed. Four flavonoid marker compounds, liquiritigenin, liquiritin, isoliquiritigenin, and isoliquiritin, from Radix Glycyrrhizae were quantitatively analyzed in this preparation. Methods The separation was performed on a reversed-phase C18 column by using a gradient elution with mobile phase of (A) water-formic acid (100∶0.04, V/V) (pH 3) and (B) acetonitrile. The mobile phase gradient was set from 0-5 min at 10% of B and 45 min to 90% of B. The assay was carried out at a flow rate of 0.2 mL·min^-1 at room temperature with the UV detection wavelengths at 280 nm and 368 nm. Results The extract (25 mg·mL^-1) of Sijunzi decoction contains 1.47 μg·mL^-1 liquiritigenin, 16.40 μg·mL^-1 liquiritin, 0.66 μg·mL^-1 isoliquiritigenin, and 2.12 μg·mL^-1 isoliquiritin. The recoveries for the sample preparation of the markers were 102.2%, 97.7%, 100.3%, and 99.9%, respectively. Conclusion The method is simple and accurate. This study reports a routine quantitative method for the analysis of multiple components in Sijunzi decoction by HPLC.

Key words: traditional Chinese medicine, traditional Chinese medicine, Sijunzi decoction, HPLC, Sijunzi decoction, HPLC

中图分类号:

R284.1

R927.2

Supporting: ^*Corresponding author. Tel.: 86-10-62899707; fax: 86-10-62898425

刘扬, 杨峻山^*. 高效液相色谱法对中药复方四君子汤中异甘草素、甘草素、异甘草苷和甘草苷的含量测定[J]. .

LIU Yang, YANG Jun-shan^*.

Determination of Liquiritigenin, Liquiritin, Isoliquiritigenin and Isoliquiritin in Extract of Traditional Chinese Medicine Sijunzi Decoction by High-Performance Liquid Chromatography

[J]. .

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