6种中药在白腐菌发酵前后降血脂作用变化研究

6种中药在白腐菌发酵前后降血脂作用变化研究

陶君彦^#, 郑国华^#, 赵雷, 黄志军, 张琼光, 邬建国, 张晓昱^*

1. 武汉大学生命科学院, 湖北武汉 430072
2. 湖北中医学院药学院, 湖北武汉 430065
3. 华中科技大学协和医学院传染科, 湖北武汉 430022
4. 武汉健民集团, 湖北武汉 430054
5. 华中科技大学生命科学与技术学院, 湖北武汉 430074

收稿日期:2009-03-02 修回日期:2009-05-10 出版日期:2009-06-15 发布日期:2009-06-15
通讯作者: 张晓昱*

The anti-hyperlipidemia activities of six herbs before and after fermentation with white rot fungi

Jun-Yan Tao^#, Guo-Hua Zheng^#, Lei Zhao, Zhi-Jun Huang, Qiong-Guang Zhang, Jian-Guo Wu, Xiao-Yu Zhang^*

1. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
2. School of Pharmacy, Hubei College of Traditional Chinese Medicine, Wuhan 430065, China
3. Department of Hepatology and Infectious Disease, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China
4. Wuhan Jianmin Pharmacy of TCM Co. Ltd., Wuhan 430054, China
5. College of Life Science & Technology, Huazhong University of Science and Technology, Wuhan 430074, China

Received:2009-03-02 Revised:2009-05-10 Online:2009-06-15 Published:2009-06-15
Contact: Xiao-Yu Zhang*

摘要/Abstract

摘要：

高血脂具有高致病性。本文通过比较中药发酵前后降血脂变化来探讨6味中药功效的变化。6味中药采用白腐菌灵芝发酵, 分成不同动物实验组, 采用洛伐他汀为阳性对照药。小鼠在连续用药15天后, 腹腔注射Triton 3393, 然后检测血清总胆固醇和总甘油三酯, 并对部分中药发酵前后的HPLC指纹图谱进行了比较。结果显示益母草发酵前, 桑枝和藤金银花发酵后, 苦参发酵前后均能降低甘油三酯, 而桑枝和益母草发酵前可以降低胆固醇含量。同时通过高效液相色谱分析发现一些新成分在发酵后产生。因此, 白腐菌发酵提高了部分中药降血脂功效, 并产生一些新的物质, 表明中药通过一些药用真菌发酵提高或改变功效的方法值得进一步探讨研究。白腐菌发酵中药功效变化研究值得进一步探索。

关键词: 降血脂, 白腐真菌, 发酵, 中药

Abstract:

Hyperlipidemia has high morbidity. We explored the novel activities of six Chinese herbs before and after fermentation for preventing hyperlipidemia. The herbs were fermented with white rot fungi Ganoderma lucidum, a strain of EN2. Lovastatin was used as the positive control. Mice were treated with those herbs before and after fermentation treatment for 15 d and then injected peritoneally with Triton 3393. After the treatment, the serum total cholesterol (TC) and triglyceride (TG) were measured. Moreover, the changes of the ingredients of those herbs were analyzed by fingerprint chromatography. The results showed that Herba leonuri before fermentation, Ramulus mori and Caulis lonicerae after fermentation, Radix sophorae flavescentis before and after fermentation showed activities to prevent hypertriglyceridemia, while Ramulus mori and Herba leonuri before fermentation showed activities to prevent hypercholesterolemia. In addition, some new ingredients were appeared after fermentation. In conclusion, the novel activities of these herbs to prevent hyperlipidemia were identified and the new ingredients that emerged after fermentation warrant further exploration.

Key words: Anti-hyperlipidemia effect, Anti-hyperlipidemia effect, White rot fungi, White rot fungi, Fermentation, Fermentation, Chinese medicine, Chinese medicine

中图分类号:

R285.5

Supporting:

^#Co-first author for the paper.

^*Corresponding author. Tel./ fax: 86-027-87792108;

e-mail: zhangxiaoyu@mail.hust.edu.cn

陶君彦^#, 郑国华^#, 赵雷, 黄志军, 张琼光, 邬建国, 张晓昱^*. 6种中药在白腐菌发酵前后降血脂作用变化研究[J]. .

Jun-Yan Tao^#, Guo-Hua Zheng^#, Lei Zhao, Zhi-Jun Huang, Qiong-Guang Zhang, Jian-Guo Wu, Xiao-Yu Zhang^* . The anti-hyperlipidemia activities of six herbs before and after fermentation with white rot fungi [J]. .

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