黄芪中主要异黄酮苷及其苷元的含量分析

黄芪中主要异黄酮苷及其苷元的含量分析

杜新刚, 白焱晶, 王邠, 赵玉英, 张庆英^*, 黄璐琦

1. 北京大学药学院天然药物学系; 天然药物及仿生药物国家重点实验室, 北京 100191;
2. 中国中医科学院中药研究所, 北京 100700

收稿日期:2008-03-15 修回日期:2008-08-10 出版日期:2008-09-15 发布日期:2008-09-15
通讯作者: 张庆英*

Analysis of principal isoflavone glycosides and aglycones in Radix astragali

Xin-Gang Du, Yan-Jing Bai, Bin Wang, Yu-Ying Zhao, Qing-Ying Zhang^*, Lu-Qi Huang

1. The State Key Laboratory of Natural and Biomimetic Drugs;
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China;
2. Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China

Received:2008-03-15 Revised:2008-08-10 Online:2008-09-15 Published:2008-09-15
Contact: Qing-Ying Zhang*

摘要/Abstract

摘要：

采用高效液相色谱-二极管阵列检测器 (HPLC/DAD) 方法测定黄芪药材和饮片中主要异黄酮苷及其苷元的含量, 并采用高效液相质谱联用技术 (LC/MSn) 鉴定主要未知色谱峰的结构。测定了黄芪药材及饮片中2个主要异黄酮苷: 毛蕊异黄酮苷 (1), 芒柄花苷 (2) 及其相应苷元毛蕊异黄酮 (3), 芒柄花素 (4)的含量, 并鉴定了两个主要未知色谱峰的结构分别为毛蕊异黄酮苷 6'''-O-丙二酸酯 (U1) 和芒柄花苷 6'''-O-丙二酸酯 (U2)。结果表明黄芪药材中异黄酮苷类成分的含量高于饮片, 而苷元含量则低于饮片。另外, 将黄芪药材粉末用水润湿后在35 ºC 放置24小时, 其中的异黄酮苷及其丙二酸酯类成分几乎全部转变成了苷元。黄芪中异黄酮苷类成分在一定条件下可转化为苷元, 异黄酮苷和苷元在黄芪药材与饮片中含量的不同可能与饮片制备过程中苷类成分的酶解有关。

关键词: 黄芪, 黄芪, 黄芪, 蒙古黄芪, 蒙古黄芪, 蒙古黄芪, 膜荚黄芪, 膜荚黄芪, 膜荚黄芪, 异黄酮, 异黄酮, 异黄酮

Abstract:

Two major isoflavone glycosides [calycosin 7-O-β-D-glucopyranoside (1) and ononin (2)] and their aglycones [calycosin (3) and formononetin (4)] were simultaneously quantified with HPLC/DAD method. Two unknown compounds were identified as calycosin 7-O-β-D-glucopyranoside-6'''-O-malonate (U1) and formononetin 7-O-β-D-glucopyranoside-6'''-O-malonate (U2), respectively, with LC/MSn. Raw Radix astragli were shown to have higher contents of isoflavone glycosides (1, 2), but lower contents of aglycones (3, 4) than the processed herbal materials. After being moistened with water and stored up for 24 h at 35 ºC, the glycosides and their malonates were almost completely transformed to their corresponding aglycones. The different contents of the isoflavone glycosides and their aglycones in raw and processed Radix astragali materials might be due to enzymolysis of the glycosides during processing.

Key words: Radix astragali, Radix astragali, Astragalus membranaceus var mongholicus, Astragalus membranaceus var mongholicus, Astragalus membranaceus, Astragalus membranaceus, Isoflavones, Isoflavones, ,

Supporting: Foundation items: National Natural Science Foundation of China (Grant No. 20432030 and 20742005).
^*Corresponding author. Tel.: 86-10-82801725;

杜新刚, 白焱晶, 王邠, 赵玉英, 张庆英^*, 黄璐琦
. 黄芪中主要异黄酮苷及其苷元的含量分析[J]. .

Xin-Gang Du, Yan-Jing Bai, Bin Wang, Yu-Ying Zhao, Qing-Ying Zhang^*, Lu-Qi Huang. Analysis of principal isoflavone glycosides and aglycones in Radix astragali
[J]. .

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