苏氨酸双铜配合物对糖尿病小鼠血糖、血脂的影响及对心肾血管的保护作用

苏氨酸双铜配合物对糖尿病小鼠血糖、血脂的影响及对心肾血管的保护作用

谢英, 王菲, 李菲菲, 李明升, 邓丽丽^*

1.北京大学药学院药剂教研室, 北京 100083;
2.北京市第十三中学, 北京 100009;
3.总参管理局医院, 北京 100088;
4.北京积水潭医院内分泌科, 北京 100035

收稿日期:2006-09-10 修回日期:2007-02-10 出版日期:2007-03-15 发布日期:2007-03-15
通讯作者: 邓丽丽*

Effects of binuclear copper(Ⅱ)threonine complex on blood glucose, lipids and protection of the hearts and kidneys in diabetic mice

Ying Xie, Fei Wang, Fei-Fei Li, Ming-Sheng Li, Li-Li Deng^*

1.Department of Pharmacy, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
2. No. 13 High School, Beijing 100009, China;
3. Hospital of Guarantee Ministry in General Staff, Beijing 100088, China;
4. Department of Endocrinology, Beijing Jishuitan Hospital, Beijing 100035, China

Received:2006-09-10 Revised:2007-02-10 Online:2007-03-15 Published:2007-03-15
Contact: Li-Li Deng*

摘要/Abstract

摘要： 目的探讨苏氨酸双铜配合物对糖尿病小鼠血糖、血脂的影响以及对心肾血管的保护作用。方法采用四氧嘧啶腹腔注射制作小鼠糖尿病模型。灌胃给与糖尿病模型小鼠低、中、高(0.002%, 0.02%, 0.1%)三种剂量的苏氨酸双铜配合物, 测定给药0, 30及45天的空腹血糖, 45天处死动物取血及心、肾组织, 测定糖化血红蛋白、血脂及观察不同剂量药物对糖尿病小鼠的心、肾病理改变的影响。结果与蒸馏水灌胃对照组比较:苏氨酸双铜配合物中剂量组降血糖作用最显著(P<0.01), 低剂量组降血糖作用次之(P<0.05); 苏氨酸双铜配合物低、中、高剂量组胆固醇(TC)水平明显降低(P<0.05), 高密度脂蛋白(HDL)水平明显升高(P<0.05); 中剂量组低密度脂蛋白(LDL)水平明显降低(P<0.05); 中剂量组心肌纤维排列整齐,细胞核和细胞浆分布均匀, 肾小球结构正常, 细胞大小分布均匀, 染色正常。结论苏氨酸双铜配合物具有显著的降血糖、调节血脂紊乱的作用, 并对糖尿病小鼠的心肾组织起到明显的保护作用, 其中以中剂量组(0.02%)效果最佳。

关键词: 苏氨酸双铜配合物, 苏氨酸双铜配合物, 苏氨酸双铜配合物, 糖尿病小鼠, 糖尿病小鼠, 糖尿病小鼠, 血糖, 血糖, 血糖, 血脂, 血脂, 血脂

Abstract: Aim To study the effects of binuclear copper (Ⅱ) threonine complex (Cu₂(Thr)₄) as analogue of superoxide dismutase (SOD) on blood glucose, blood lipids and vessels of hearts and kidneys in diabetic mice. Methods Diabetic mouse model was established by intraperitioneal injection of alloxan. Low, middle, and high doses of Cu₂(Thr)₄ at 0.002%, 0.02% and 0.1% were given respectively to diabetic mice following lavage. The fasting blood glucose was determined after the diabetic mice were given Cu₂(Thr)₄ for 0, 30, and 45 d. The diabetic mice were killed on the 45th day. Then glycosylated hemoglobin(HbA1c) and blood lipids were assayed, and pathologic changes in hearts and kidneys stained with HE were observed. Results Compared with the control group in which the diabetic mice were given distilled water, the value of blood glucose reduced significantly in middle dose group (P< 0.01), followed by that in low dose group(P< 0.05). TC level reduced markedly and HDL level increased significantly in all three treatment groups (P< 0.05). Especially in middle dose group, cardiac muscle fibers were neatly arranged, nucleus and cytoplasm well distributed, glomeruli showing normal structure, cells well distributed and staining being normal. Conclusion Cu₂(Thr)₄ reduces blood glucose, regulates blood lipids, and play protective action on the vessels of hearts and kidneys in diabetic mice. The effects of it in middle dose were better than those of other doses.

Key words: Binuclear copper Ⅱ threonine complex, Binuclear copper Ⅱ threonine complex, Diabetic mice, Diabetic mice, Blood glucose, Blood glucose, Blood lipids, Blood lipids

中图分类号:

R913
R916.2

Supporting:

谢英, 王菲, 李菲菲, 李明升, 邓丽丽^*

. 苏氨酸双铜配合物对糖尿病小鼠血糖、血脂的影响及对心肾血管的保护作用[J]. .

Ying Xie, Fei Wang, Fei-Fei Li, Ming-Sheng Li, Li-Li Deng^*. Effects of binuclear copper(Ⅱ)threonine complex on blood glucose, lipids and protection of the hearts and kidneys in diabetic mice[J]. .

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