宽叶羌活根茎和根的挥发油成分的GC-MS分析

宽叶羌活根茎和根的挥发油成分的GC-MS分析

杨秀伟^*, 张鹏, 陶海燕, 蒋舜媛, 周毅

1.北京大学药学院天然药物及仿生药物国家重点实验室, 北京 100083;
2.四川省中药研究所, 四川成都 610041

收稿日期:2006-07-17 修回日期:2006-11-10 出版日期:2006-12-15 发布日期:2006-12-15
通讯作者: 杨秀伟*

GC-MS Analysis of Essential Oil Constituents from Rhizome and Root of Notopterygium forbesii

YANG Xiu-wei^*, ZHANG Peng, TAO Hai-yan, JIANG Shun-yuan, ZHOU Yi

1.The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
2.Sichuan Institute of Chinese Materia Medica, Chengdu 610041, China

Received:2006-07-17 Revised:2006-11-10 Online:2006-12-15 Published:2006-12-15
Contact: YANG Xiu-wei*

摘要/Abstract

摘要： 目的分析宽叶羌活(Notopterygium forbesii Boiss.)根茎和根的挥发油化学成分, 为其质量评价提供科学依据。方法采用水蒸气蒸馏法提取宽叶羌活根茎和根的挥发油, 用GC毛细管柱进行分析,归一化法测定其相对含量, 并用GC-MS法鉴定化学成分。结果共检出217个色谱峰, 初步鉴定出100个化合物, 占挥发油总量的78.30%。结论宽叶羌活根茎和根的挥发油中的化学成分主要为单萜和倍半萜类化合物, 两者分别占总挥发油中化学成分的41.30%和32.65%。这与狭叶羌活根茎和根的挥发油中单萜和倍半萜类化合物分别占总挥发油中化学成分的13.63%和67.93%不同。(1R)-α-蒎烯(3.98%)、桧烯(2.51%)、(1S)-β-蒎烯(5.78%)、m-月桂烯(4.05%)、柠檬烯(3.55%)、3,7-二甲基-1,3,7-庚三烯(3.98%)、γ-萜品烯(8.70%)、茴香醚(1.78%)和(1S)-桥环醋酸冰片酯(2.62%)为单萜类化合物及其衍生物中的主要成分; (+)-β-榄香烯(1.17%)、异愈创木烯(1.40%)、(+)-β-没药烯(1.21%)、(±)-榄香醇(1.42%)、愈创木醇(1.14%)、去羟基异菖蒲二醇(1.13%)、(-)-土青木香烯(1.99%)、异愈创木醇(9.48%)、γ-桉醇(1.77%)、愈创木-1(10)-烯-11-醇(1.12%)和α-没药醇(5.35%)为倍半萜类化合物及其衍生物中的主要成分。

关键词: 伞形科, 伞形科, 伞形科, 宽叶羌活, 宽叶羌活, 宽叶羌活, 挥发油, 挥发油, 挥发油, 毛细管色谱, 毛细管色谱, 毛细管色谱, 气相色谱-质谱, 气相色谱-质谱, 气相色谱-质谱

Abstract: Aim To analyse the chemical constituents of the essential oil extracted from the rhizome and root of Notopterygium forbesii Boiss. and provide scientific basis for quality control. Methods The total essential oil was extracted by water-steam distillation and separated by capillary gas chromatography (GC). The components were determined by normalization method, and identified by GC-MS. Results GC-MS exhibited 217 peaks and 100 compounds were identified, accounting for 78.3% of the total essential oil. Conclusion In the total essential oil of the rhizome and root of N. forbesii, monoterpenes and sesquiterpenes account for 41.30% and 32.65%, respectively, in which (1R)-α-pinene (3.98%), sabinene (2.51%), (1S)-β-pinene (5.78%), m-cymene (4.05%), limonene (3.55%), 3, 7-dimethyl-1, 3, 7-octatriene (3.98%), γ-terpinene (8.70%), anisole (1.78%) and (1S)-endo-bornyl acetate (2.62%) as the monoterpenes and its derivatives, and (+)-β-elemene (1.17%), α-bulnesene (1.40%), (+)-β-bisabolene (1.21%), (±)-elemol (1.42%), guaiol (1.14%), dehydroxyisocalamendiol (1.13%), (-)-aristolene (1.99%), bulnesol (9.48%), γ-eudesmol (1.77%), guai-1 (10)-en-11-ol (1.12%) and α-bisabolol (5.35 %) as the sesquiterpenes and its derivatives are the main components.

Key words: Umbelliferae, Umbelliferae, Notopterygium forbesii, Notopterygium forbesii, essential oil, essential oil, capillary gas chromatography, capillary gas chromatography, GC-MS, GC-MS

中图分类号:

R284.1

R917.1

Supporting: Foundation item: State Projects of the Tenth-Five-Year Plan (No.
2001BA701A60203)
^*Corresponding author. Tel.: 86-10-82805106, 86-10-82801569

杨秀伟^*, 张鹏, 陶海燕, 蒋舜媛, 周毅. 宽叶羌活根茎和根的挥发油成分的GC-MS分析[J]. .

YANG Xiu-wei^*, ZHANG Peng, TAO Hai-yan, JIANG Shun-yuan, ZHOU Yi . GC-MS Analysis of Essential Oil Constituents from Rhizome and Root of Notopterygium forbesii[J]. .

参考文献

[1] Yang XW, Yan ZK, Gu ZM, et al. Studies on the chemical constituents of forbes Notopterygium (Notopterygium forbesii) [J]. Chin Pharm J, 1994, 29 (3): 141–143.
[2] Yang XW, Yan ZK, Gu ZM, et al. Studies on the chemical constituents of incised Notopterygium (Notopterygium incisum ) [J]. Chin Tradit Herb Drugs, 1993, 24 (10): 507–511.
[3] Gu ZM, ZhangDX, Yang XW, et al. Isolation of two new coumarin glycosides from N otopterygium forbesii and evaluation of a Chinese crude drug, Qiang-huo, the underground parts of N. incisum and N. forbesii, by high-performance liquid chromatography [J]. Chem Pharm Bull, 1990, 38 (9): 2498–2502.
[4] Yang XW, Zhang P, Tao HY, et al. GC-MS analysis of essential oil constituents from the rhizome and root of Notopterygium incisum [J]. J Chin Pharm Sci, 2006, 15 (3): 172–177.
[5] State Pharmacopoeia Committee of the People’s Republic of China. Pharm acopoeia of the People’s Republic of China [S]. 2000 Ed. Beijing: Chemical Industry Press, 2000. Appendix: 64.
[6] Grassmann J. Terpenoids as p lant antioxidants [J]. Vitam Horm, 2005, 72: 505–535.
[7] CalcabriniA, Stringaro A, ToccacieliL, et al. Terpinen-4-ol, the main component of M elaleuca alternifolia (tea tree) oil inhibits the in vitro growth of human melanoma cells [J]. J Invest Derm atol, 2004, 122 (2): 349–360.
[8] Budhiraja SS, Cullum ME, Sioutis SS, et al. Biological activity of M elaleuca alternifolia ( tea tree ) oil component, terpinen-4-ol, in human myelocytic cell line HL-60 [J]. J M anipulative Physiol Ther, 1999, 22 (7): 447–453.
[9] Hart PH, Brand C, Carson CF, et al. Terpinen-4-ol, the main component of the essential oil of Melaleuca alternifolia (tea tree oil), suppresses inflammatory mediator production by activated human monocytes [J]. Inflamm Res, 2000, 49 (11): 619–626.
[10] De-Oliveira AC, Ribeiro-Pinto LF, Paumgartten JR. In vitro inhibition of CYP2B1 monooxygenase by beta-myrcene and other mono terpenoid compounds [J]. Tox icol Lett, 1997, 92 (1): 39–46.
[11] Asakura K, Kanemasa T, Minagawa K, et al. alpha-Eudesmol, a P/Q-type Ca²⁺ channel blocker, inhibits neurogenic vasodilation and extravasation following electrical stimulation of trigeminal ganglion [J]. Brain Res, 2000, 873 (1): 94–101.
[12] Asakura K, Matsuo Y, Oshima T, et al. omega-Agatoxin IVA-sensitive Ca²⁺channel blocker, alpha-eudesmol, protects against brain injury after focal ischemia in rats [J]. Eur J Pharm acol, 2000, 394: 57–65.
[13] Kimura I. Medical benefits of using natural compounds and their derivatives having multip le pharmacological actions [J]. Yakugaku Zasshi, 2006, 126 (3): 133–143.
[14] Hsieh TJ, Chang FR, Chia YC, et al. Cytotoxic constituents of the fruits of Cananga odorata [J]. J N at Prod, 2001, 64 (5): 616–619.