RP-HPLC法测定天葵子中格列风内酯的含量

RP-HPLC法测定天葵子中格列风内酯的含量

牛锋, 谢光波, 崔征, 陈发奎, 屠鹏飞^*

1.北京大学药学院天然药物学系, 北京 100083;
2.沈阳药科大学中药学院生药教研室, 辽宁沈阳 110016

收稿日期:2006-04-28 修回日期:2006-08-10 出版日期:2006-09-15 发布日期:2006-09-15
通讯作者: 屠鹏飞*

Determination of Griffonilide in Roots of Semiaquilegia adoxoides by RP-HPLC

NIU Feng, XIE Guang-bo, CUI Zheng, CHEN Fa-kui, TU Peng-fei^*

1.Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
2.Department of Pharmacognosy, School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China

Received:2006-04-28 Revised:2006-08-10 Online:2006-09-15 Published:2006-09-15
Contact: TU Peng-fei*

摘要/Abstract

摘要： 目的建立使用反相高效液相法测定天葵子药材中苯骈呋喃酮型内酯格列风内酯含量的方法。方法使用迪马Kromasil ODS柱, 流动相为甲醇-水(含0.01%三氟醋酸, 0.05%四氢呋喃) (3:97), 检测波长为260 nm。结果在0.004 – 0.4 mg·mL^-1的范围内, 峰面积与浓度线性良好, 相关系数为r = 1.000 00; 平均回收率为98.77%; RSD =2.77%。结论本文对天葵子药材进行了质量控制方面的研究, 并首次报道了格列风内酯的含量测定方法。本法快速、准确、灵敏度高, 可以作为天葵子药材中格列风内酯含量测定的方法。

关键词: 天葵, 天葵, 天葵, 天葵子, 天葵子, 天葵子, 苯骈呋喃酮, 苯骈呋喃酮, 苯骈呋喃酮, 格列风内酯, 格列风内酯, 格列风内酯, RP-HPLC, RP-HPLC, RP-HPLC

Abstract: Aim To establish the method for determination of benzofuranones derivative, griffon-ilide, in the roots of Semiaquilegia adoxoides (DC.) Makino by RP- HPLC. Methods Kromasil C₁₈column was used with a mobile phase of methanol-water (containing 0.01% trifluoroacetic acid and 0.05% tetrahydrofuran) (3:97). The detecting wavelength was 260 nm. Results The calibration curve was linear over the range of 0.004 – 0.4 mg·mL^-1 with the correlation coefficient of 1.00 000. The average recovery was 98.77% with RSD 2.77%. Determinations of ten samples from different areas showed that the contents of griffonilide in the roots of S.adoxoides are 0.17 – 0.49 mg·g^-1. Conclusion The method provides an accurate and sensitive way to detect griffonilide in the roots of S.adoxoides.

Key words: Semiaquilegia adoxoides, Semiaquilegia adoxoides, benzofuranones derivative, benzofuranones derivative, griffonilide, griffonilide, RP-HPLC, RP-HPLC

中图分类号:

R284.1

R917.01

Supporting: Foundation item: Ministry of Science and Technology of Peop le's Republic of China (No. 2004AA2Z3730)
^*Corresponding author. Tel.: 86-10-82802750

牛锋, 谢光波, 崔征, 陈发奎, 屠鹏飞^*. RP-HPLC法测定天葵子中格列风内酯的含量[J]. .

NIU Feng, XIE Guang-bo, CUI Zheng, CHEN Fa-kui, TU Peng-fei^*. Determination of Griffonilide in Roots of Semiaquilegia adoxoides by RP-HPLC[J]. .

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