狭叶羌活根茎和根的挥发油成分的GC-MS分析

狭叶羌活根茎和根的挥发油成分的GC-MS分析

杨秀伟^*, 张鹏, 陶海燕, 蒋舜媛, 周毅

1.北京大学药学院天然药物及仿生药物国家重点实验室, 北京 100083;
2.四川省中药研究所, 四川成都 610041

收稿日期:2006-04-20 修回日期:2006-08-10 出版日期:2006-09-15 发布日期:2006-09-15
通讯作者: 杨秀伟*

GC-MS Analysis of Essential Oil Constituents from Rhizome and Root of Notopterygium incisum

YANG Xiu-wei^*, ZHANG Peng, TAO Hai-yan, JIANG Shun-yuan, ZHOU Yi

1.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
2. Sichuan Institute of Chinese Materia Medica, Chengdu 610041, China

Received:2006-04-20 Revised:2006-08-10 Online:2006-09-15 Published:2006-09-15
Contact: YANG Xiu-wei*

摘要/Abstract

摘要： 目的分析狭叶羌活(Notopterygium incisum Ting ex H. T. Chang)根茎和根的挥发油的化学成分, 为其质量评价提供科学依据。方法采用水蒸气蒸馏法提取狭叶羌活根茎和根的挥发油, 用GC毛细管柱进行分析, 归一化法测定其相对含量, 并用GC-MS法鉴定化学成分。结果检出242个色谱峰, 初步鉴定出83个化合物, 占挥发油总量的75.77%。结论狭叶羌活根茎和根的挥发油中的化学成分主要为单萜和倍半萜类化合物, 两者分别占总油中化学成分的13.63 %和67.93 %。(1S)-β-蒎烯(1.67%)、3-蒈烯(1.05%)、柠檬烯(1.22%)和1S-endo-醋酸冰片酯(1.68%)为单萜类化合物中的主要成分; ( +)-β-榄香烯(6.78%)、苜蓿烯(1.54%)、α-石竹烯(2.64%)、异大香叶烯D (1.67%)、桉烷-4 (14), 11-二烯(2.36%)、α-芹子烯(2.42%)、δ-杜松烯(1.55%)、3, 7, 11-三甲基-2, 6, 10-十二烷三烯-1-醇(1.03%)、(±)-榄香烯(5.18)、(-)-匙叶桉油烯醇(1.40%)、愈创醇(3.81%)、去羟基异菖蒲烯二醇(1.06%)、γ-桉醇(1.05%)、α-桉醇(7.97%)、异愈创木醇(3.09%)和胡萝卜醇(2.30%)为倍半萜类化合物中的主要成分。总挥发油中, 反式肉桂酸异丙酯占11.3%, 为所有化合物中含量最高者。,

关键词: 伞形科, 伞形科, 伞形科, 狭叶羌活, 狭叶羌活, 狭叶羌活, 挥发油, 挥发油, 挥发油, 毛细管色谱, 毛细管色谱, 毛细管色谱, 气相色谱-质谱, 气相色谱-质谱, 气相色谱-质谱

Abstract: Aim To analyse the chemical constituents of the essential oil extracted from the rhizome and root of Notopterygium incisum Ting ex H.T. Chang,providingscientific basis for quality control. Methods The total essential oil was extracted by water-steam distillation and separated by capillary gas chromatography (GC). The components were determined bynormalization method, and identified by GC-MS. Results GC-MS exhibited 242 peaks and 83 compounds were identified, accounting for 75.77% of the total essential oil. Conclusion In the total essential oil of the rhizome and root of N. incisum, monoterpenes and sesquiterpenes accountedfor 13.63% and 67.93%, respectively, in which (1S)-β-pinene (1.67%), 3-carene (1.05%), limonene (1.22%), and 1S-endo-bornyl acetate (1.68%) as the monoterpenes and its derivatives, and (+)-β-elemene(6.78%), sativene(1.54%), α-caryophyllene (2.64%), germacrene D (1.67%), eudesma-4 (14),11-diene(2.36%), α-selinene(2.42%), δ-cadinene(1.55%), 3,7,11-trimethyl-2,6,10-dodecatrien-1-ol (1.03%), (±)-elemol (5.18%), (-)-spathulenol (1.40%), guaiol (3.81%), dehydroxy-isocalamendiol (1.06%), γ-eudesmol (1.05%), α-eudesmol (7.97%), bulnesol (3.09%),and carotol (2.30%) as the sesquiterpenes and its derivatives were main components. In addition, isopropyl trans-cinnamatewas the maximum compound (11.3%) of the total essential oil.

Key words: Umbelliferae, Umbelliferae, Notopterygium incisum, Notopterygium incisum, essential oil, essential oil, capillary gas chromatography, capillary gas chromatography, GC-MS, GC-MS

中图分类号:

R28411

R917.1

Supporting: Foundation item: State Projects of the Tenth-Five-Year Plan (No.2001BA701A60203).
Corresponding author. Tel.: 86-10-82805106, 86-10-82801569

杨秀伟^*, 张鹏, 陶海燕, 蒋舜媛, 周毅. 狭叶羌活根茎和根的挥发油成分的GC-MS分析[J]. .

YANG Xiu-wei^*, ZHANG Peng, TAO Hai-yan, JIANG Shun-yuan, ZHOU Yi. GC-MS Analysis of Essential Oil Constituents from Rhizome and Root of Notopterygium incisum[J]. .

参考文献

[1] Yang XW, Yan ZK, Gu ZM, et al. Studies on the chemical constituents of forbes Notopterygium (Notopterygium forbesii) [J]. Chin Pharm J, 1994, 29 (3) : 141–143.
[2] Yang XW, Yan ZK, Gu ZM, et al. Studies on the chemical constituents of incised Notopterygium (Notopterygium incisum) [J]. Chin Tradit Herb Drugs, 1993, 24 (10) : 507–511.
[3] Gu ZM, Zhang DX, Yang XW, et al. Isolation of two new coumarin glycosides from Notopterygium forbesii and evaluation of a Chinese crude drug, Qiang-huo, the underground parts of N. incisum and N. forbesii, by high-performance liquid chromatography [J]. Chem Pharm Bull, 1990, 38 (9) : 2498–2502.
[4] Yang XW, Gu ZM, Wang BX, et al. Comparison of antilip id peroxidative effects of the underground parts of Notopterygium incisum and N. forbesii in mice [J]. Planta Med, 1991, 57: 399–401.
[5] Gao GH, Deng JY, Wang XM, et al. Quality assessment of rhizomaet Radix Notopterygii by the gas chromatographic fingerprint [J]. J Shenyang Pharm Univ, 2004, 21 (6) : 438–441.
[6] Shan Y, Fan JF. Compared studies on the essential components of Can-Qiang and Jitui-Qiang [J]. China J Chin MaterMed, 1998, 23 (2) : 100–102.
[7] Fan JF, L iu D, Sun YF, et al. Study of chemical constituents of Qiang-huo (Notopterygium incisum Ting) I. isolation and identification of essential oil in Qiang-Huo [J]. Bull Chin Mater Med, 1981, 6 (1) : 29–30.
[8] Chinese Pharmacopoeia, 2000 ed. Vol. 1 [S]. Beijing: Chemical Industry press. (Appendix) 64.
[9] Asakura K, Kanemasa T, Minagawa K, et al. Alpha-eudesmol, a P/Q-type Ca²⁺ channel blocker, inhibits neurogenic vasodilation and extravasation following electrical stimulation of trigeminal ganglion [J]. Brain Res, 2000, 873 (1) : 94–101.
[10] Asakura K, Matsuo Y, Oshima T, et al. Omega-agatoxin IVA-sensitive Ca²⁺ channel blocker, alpha-eudesmol, protects against brain injury after focal ischemia in rats [J]. Eur J Pharmacol, 2000, 394: 57–65.
[11] Kimura I. Medical benefits of using natural compounds and their derivatives having multiple pharmacological actions [J]. Yakugaku Zasshi, 2006, 126 (3) : 133–143.
[12] Hsieh TJ , Chang FR, Chia YC, et al. Cytotoxic constituents of the fruits of Cananga odorata [J]. J Nat Prod, 2001, 64 (5) : 616–619.