利用大孔树脂从葛根中分离纯化总黄酮

利用大孔树脂从葛根中分离纯化总黄酮

刘火安, 王伯初, 戴传云^*, 邵志勇, 何从林, 贾云

1.重庆大学生物工程学院, 重庆 400030;
2.重庆科技学院化学与生物工程学院, 重庆
3.Interdepartment of Genetics, Iowa State University, Iowa 50011, USA

收稿日期:2005-09-25 修回日期:2006-05-10 出版日期:2006-06-15 发布日期:2006-06-15
通讯作者: 戴传云*

Optimization of Preparative Separation and Purification of Total Flavonoids from Radix Puerariae by Macroporous Resin Method

LIU Huo-an, WANG Bo-chu, DAI Chuan-yun^*, SHAO Zhi-yong, HE Cong-lin, JIA Yun

1.College of Bioengineering, Chongqing University, Chongqing 400030, China;
2.College of Chemistry and Biology, Chongqing Polytechnic College, Chongqing 400050, China;
3. Interdepartment of Genetics, Iowa State University, Iowa 50011, USA

Received:2005-09-25 Revised:2006-05-10 Online:2006-06-15 Published:2006-06-15
Contact: DAI Chuan-yun*

摘要/Abstract

摘要： 目的筛选分离葛根总黄酮的最佳树脂, 并对影响分离的各种因素进行系统的研究, 使纯化工艺达到最优。方法采用静态与动态的吸附-解吸两种方法, 利用紫外可见分光光度计测量葛根总黄酮的含量, 研究不同大孔吸附树脂及其不同的工艺条件对总黄酮分离纯化的影响。结果 SP70分离效果最好, 其最佳工艺为药液浓度0.5 g·mL^-1 (相当于原生药)、pH为5-6、以2 BV·h^-1速率进行上样, 上样量为60 BV, 以5 BV的70%乙醇、2 BV·h^-1的流速进行洗脱, 效果最佳。经SP70处理后的葛根总黄酮的含量可达80%以上。结论大孔吸附树脂SP70分离纯化总黄酮效果较好, 适合工业生产。

关键词: 葛根, 葛根, 葛根, 总黄酮, 总黄酮, 总黄酮, 大孔树脂, 大孔树脂, 大孔树脂, 分离与纯化, 分离与纯化, 分离与纯化

Abstract: Aim To screen the optimum macroporous resin and conditions for the isolation and purification of flavonoids from Radix Puerariae. Methods The static and dynamic adsorption/desorption methods were used, and the separation and purification process was evaluated by measuring the concentration of total flavonoid in the fractions with UV spectrophotometer. Results The SP70 macroporous resin was the most effective compared with other macroporous resins. The optimum conditions were screened, which were 0.5 g·mL^-1 corresponding to crude drug for concentration of extract, pH 5-6 , and appended 60 times the volume of the resin bed (BV) with the adsorption speed 2 BV·h^-1, and the volume of aq. 70% (V/V) ethanol as eluant was 5 BV with desorption speed 2 BV·h^-1. By this method, the final contents of total flavonoids exceeded 80%. Conclusion The SP70 macroporous resin is the most effective one for large-scale isolation and purification of flavonoids from Radix Pueraria, which meets industrial needs.

Key words: Radix Puerariae, Radix Puerariae, total flavonoids, total flavonoids, macroporous resin, macroporous resin, separation, separation, purification, purification

中图分类号:

R284.2

Supporting:

Foundation item: Science and Technology Committee of Chongqing in China (CSTC. 2004BB5122).
^*Corresponding author. Tel.: 86-23-89126756

刘火安, 王伯初, 戴传云^*, 邵志勇, 何从林, 贾云. 利用大孔树脂从葛根中分离纯化总黄酮[J]. .

LIU Huo-an, WANG Bo-chu, DAI Chuan-yun^*, SHAO Zhi-yong, HE Cong-lin, JIA Yun. Optimization of Preparative Separation and Purification of Total Flavonoids from Radix Puerariae by Macroporous Resin Method[J]. .

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