红球菌 Rhodococcus sp. 对葛根素的选择性乙酰化

doi:10.5246/jcps.2012.03.035

红球菌 Rhodococcus sp. 对葛根素的选择性乙酰化

丁雅光, 张德武, 刘树民, 戴均贵^*

1. 中国医学科学院北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室, 北京 100050
2. 黑龙江中医药大学中医药研究院, 黑龙江哈尔滨 150040

收稿日期:2012-02-13 修回日期:2012-04-10 出版日期:2012-05-15 发布日期:2012-05-15
通讯作者: 戴均贵*

Selective acetylation of puerarin by Rhodococcus sp.

Ya-Guang Ding, De-Wu Zhang, Shu-Min Liu, Jun-Gui Dai^*

1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
2. College of Traditional Chinese Medicine, Heilongjiang University of Traditional Chinese Medicine, Haerbin 150040, China

Received:2012-02-13 Revised:2012-04-10 Online:2012-05-15 Published:2012-05-15
Contact: Jun-Gui Dai*

摘要/Abstract

摘要：

在所试验的39株微生物中, 发现红球菌 (Rhodococcus sp. AS 4.1147) 能在葛根素 (1) 葡萄糖基的6位上进行选择性乙酰化。通过质谱和核磁共振波谱对乙酰化产物6''-O-acetylpuerarin (2) 的结构进行了确定。转化产物的分离得率为22.2%。

关键词: 葛根素, 选择性乙酰化, 红球菌Rhodococcus sp. AS 4.1147

Abstract:

Among 39 species of microbial strains, Rhodococcus sp. AS 4.1147 possessed the ability to selectively acetylate puerarin (1) at C-6 position of the glucosyl moiety. The structure of the acetylated product, 6''-O-acetylpuerarin (2) was determined by the analysis of MS, NMR spectroscopic data. The isolated yield of 2 was 22.2%.

Key words: Puerarin, Selective acetylation, Rhodococcus sp. AS 4.1147

中图分类号:

R282.6

Supporting:

Foundation items: The Science & Technology Project of Guangdong Province (Grant No. 2011A080403020), and the Program for New Century Excellent Talents in University (Grant No. NCET-06-0155).
*Corresponding author. Tel.: 86-10-63165195

丁雅光, 张德武, 刘树民, 戴均贵^*. 红球菌 Rhodococcus sp. 对葛根素的选择性乙酰化[J]. , DOI: 10.5246/jcps.2012.03.035.

Ya-Guang Ding, De-Wu Zhang, Shu-Min Liu, Jun-Gui Dai^* . Selective acetylation of puerarin by Rhodococcus sp. [J]. , DOI: 10.5246/jcps.2012.03.035.

[1]	李艳姣, 刘慧敏, 党晓芳, 张硕峰, 吴金英, 贾占红, 韩立炜^*. HPLC方法研究葛根素混合胶束在Beagle犬体内的药代动力学[J]. , 2012, 21(4): 327-332.
[2]	斯建勇, 常琪, 沈连钢, 陈迪华^*. 野葛化学成分研究[J]. , 2006, 15(4): 248-250.
[3]	翟光喜^*, 娄红祥, 毕殿洲, 邹立家. 固体分散体中葛根素与磷脂的相互作用[J]. , 2003, 12(1): 36-40.