茶多酚对脑缺血再灌注损伤的保护作用及在体外抗脂质过氧化和清除自由基作用

茶多酚对脑缺血再灌注损伤的保护作用及在体外抗脂质过氧化和清除自由基作用

何冰, 陈小夏, 陈一岳

广东药学院药理教研室, 广州 510224

收稿日期:2002-04-15 修回日期:2002-09-02 出版日期:2002-12-15 发布日期:2002-12-15

Protective Effects of Tea Polyphenol on Cerebral Ischemia-Reperfusion Injury in Rats and Its Scavenging Oxy-radical and Anticerebral Lipid Peroxidation Effects

He Bing, Chen Xiaoxia, Chen Yiyue

Deptartment of Pharmacology, Guangdong College of Pharmacy, Guangzhou 510224

Received:2002-04-15 Revised:2002-09-02 Online:2002-12-15 Published:2002-12-15

摘要/Abstract

摘要：

目的: 研究茶多酚对脑缺血再灌注损伤的保护作用及抗脂质过氧化和清除自由基的作用。方法: 结扎大鼠双侧颈总动脉及迷走神经后, 再灌注4.5min, 建立脑缺血再灌注模型。由自由基诱导的脑线粒体脂质过氧化, 用硫代巴比妥酸法测定。由Xan XO诱导的O2^- and由Fe²⁺-H2O2 诱导的·OH ,用紫外分光光度仪测定。结果: 在脑缺血再灌注损伤时, 茶多酚能改善SOD、GSH-Px、CAT活性(P<0.01), 同时降低脑水肿和MDA含量(P<0.01和P<0.05 )。茶多酚具有明显的清除·OH和O2^-的作用(IC50分别为2.2mmol·L^-1和1.9mmol·L^-1), 茶多酚明显抑制由·OH诱导脑线粒体脂质过氧化, 并呈浓度依赖性。结论: 结果提示茶多酚能保护脑缺血再灌注损伤,与其清除自由基和抗脂质过氧化作用有关.

关键词: 茶多酚, 抗脂质过氧化, 脑水肿, 脑缺血再灌注

Abstract: AIM To study the protective effects of tea polyphenol (TP) on cerebral ischemia reperfusion injury in rats and its scavenging oxygen free radical(OFR) activities and antilipid peroxidation in vitro. METHODS Cerebral ischemia reperfusion injury was produced by bilateral ligation of the common carotid arteries with vagus nerves and reperfusion for 45 min. The mitochondrial lipid peroxidation of rat brain induced by oxygen free radical was measured by thiobarbituric acid spectrophotometry. Superoxide anion (O2^-) from xanthine-xanthine oxidase system and hydroxyl radical (·OH) from Fe²⁺-H2O2 system were determined with spectrophotometry. RESULTS During Cerebral ischemia reperfusion,TP improved the activities of superoxide dismutase (P<0.05), GSH peroxidase(P<0.01) and catalase(P<0.01), while decreasing the maiondialdchyde content in the brain(P<0.05) and brain water content (P<0.01). Tea polyphenol possessed significantly scavenging effects on ·OH produced by Fenton reaction and O2^- produced by xanthine-xanthine oxidase system (the IC50 were 2.2 mmol·L^-1 and 1.9 mmol·L^-1 respectively). Tea polyphenol could significant inhibit the lipid peroxidation of cerbral mitochondrial membrane induced by ·OH in a concentration dependent manner. CONCLUSION The results indicate that tea polyphenol could protect the injury on cerebral ischemia reperfusion in rats for OFR, these effects may be related to its scavenging effects on oxygen free radicals and antilipid peroxidant.

Key words: Tea polyphenol, Tea polyphenol, Scavenging oxygen free redicals, Scavenging oxygen free redicals, Antilipid peroxidation, Antilipid peroxidation, Ischemia reperfusion, Ischemia reperfusion

Supporting:

何冰, 陈小夏, 陈一岳. 茶多酚对脑缺血再灌注损伤的保护作用及在体外抗脂质过氧化和清除自由基作用[J]. .

He Bing, Chen Xiaoxia, Chen Yiyue . Protective Effects of Tea Polyphenol on Cerebral Ischemia-Reperfusion Injury in Rats and Its Scavenging Oxy-radical and Anticerebral Lipid Peroxidation Effects[J]. .

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[6]	张精亮, 胡涛, 刘晓岩, 朱元军, 陈晓玲, 刘晔, 王银叶. 002C-3通过抑制MMPs和细胞自噬并激活细胞存活相关的钙信号通路保护小鼠脑缺血再灌注损伤[J]. 中国药学(英文版), 2016, 25(8): 598-604.
[7]	刘晓岩, 胡振宇, 陈世忠, 王坚成, 王银叶. 口服和厚朴酚对大鼠脑缺血再灌注(I-R)损伤和SHRsp脑卒中的保护作用[J]. 中国药学(英文版), 2016, 25(12): 882-891.
[8]	赵月斌, 施喆, 叶丽华, 刘会雪^, 杨晓达^, 王夔. 槲皮素和茶多酚抑制VO(acac)₂导致的线粒体损伤和细胞毒性 [J]. , 2009, 18(3): 225-231.
[9]	陈小夏, 何冰. 灯盏花素对大鼠脑缺血再灌注引起抗氧化酶活性改变的影响[J]. , 1998, 7(2): 91-93.
[10]	杨秀伟, 服部征雄, 难波恒雄. 木桔根甲醇提取物和其化学成分对小鼠在体和离体肝脏组织脂质过氧化的抑制作用的研究[J]. , 1996, 5(3): 132-140.
[11]	杨秀伟, 陈道峰, 徐国钧, 服部征雄, 难波恒雄. 地血香乙醇提取物对小鼠肝细胞中抗氧化酶活性的影响[J]. , 1994, 3(2): 147-151.