http://jcps.bjmu.edu.cn

• 研究论文 • 上一篇    下一篇

丁基苯酞对缺血脑组织中MDA形成及超氧阴离子自由基产生的抑制作用

种兆忠, 冯亦璞   

  1. 中国医学科学院,中国协和医科大学药物研究所, 北京 100050
  • 收稿日期:1998-07-10 修回日期:1998-11-24 出版日期:1999-06-15 发布日期:1999-06-15

Effect of 3-n-Butylphthalide on Reperfusion induced Lipid Peroxidation Following Cerebral Ischemia in Rats and Superoxide Radical Formation In Vitro

Chong Zhaozhong, Feng Yipu   

  1. Department of Pharmacology,Institute of Materia Medtca, Chinese Academy of Medical Sciences and Peking Union MedicalCollege, Beijing 100050
  • Received:1998-07-10 Revised:1998-11-24 Online:1999-06-15 Published:1999-06-15

摘要: 局灶性脑缺血6小时再灌注1小时后, 缺血脑组织中脂质过氧化产物雨二醛(MDA)的含量明显增加, 表明再灌注导致了脂质过氧化损伤。丁基苯酞(dl-NBP, 20 mg·kg-1, ip)可显著降低缺血皮层组织中MDA的含量。进一步研究表明, dl-NBP, d-NBPl-NBP皆可抑制黄嘌呤-黄嘌呤氧化酶反应系统中超氧阴离子自由基的形成, 此作用可能与其抑制了黄嘌呤氧化酶活性有关。

关键词: 丁基苯酞, 脑缺血, 丙二醛, 超氧阴离子, 黄嘌呤氧化酶

Abstract: Reperfusion after focal cerebral ischemia induced an increase in the content of malondialdehyde (MDA), a product of lipid peroxidation, in ischemic brain. dl-3-n-Butylphthalide (dl-NBP, 20 mg.kg-1, ip). was shown to decrease the content of MDA in cerebral cortex of rats subjected to 1 h reperfusion following 6 h MCAO. dl-NBP, d-NBP and l-NBP were also shown to inhibit the production of superoxide boon in xanthine-xanthine oxidase reaction system in vitro. The effect of dl-NBP, d-NBP and l-NBP on superoxide anion may be due to their capability of inhibiting the activity of xanthine oxidase. The results suggest that dl-NBP may possess inhibiting effect on the formation of superoxide anion and reducing effect on lipid peroxidation during reperfusion after cerebral ischemia.

Key words: dl-3-n-Butylphthalide, Cerebral ischemia, Malondialdehyde, Superoxide anion, Xanthine oxidase

Supporting:

The study was supported by a grant from State Science and Technology Commission of China. No. 94-ZD-01.