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灵芝对小鼠巨噬细胞产生肿瘤坏死因子的影响

贾永锋, 力弘, 森昌夫, 张罗修   

  1. 1. 上海医科大学药学院药理教研室, 上海 200032;
    2. 日本和汉生药研究所, 东京
  • 收稿日期:1996-10-21 修回日期:1997-09-16 出版日期:1998-03-15 发布日期:1998-03-15

Tumor Necrosis Factor Production in Mouse Macrophages and its Pharmacological Modulation by Ganoderma lucidum

Yong-Feng Jia, Hong Li, Masao Mori, Luo-Xiu Zhang   

  1. 1. Department of Pharmacology, School of Pharmacy, Shanghai Medical University, Shanghai 200032;
    2. Wakan Shoyaku Botany Institute, Tokyo, Japan
  • Received:1996-10-21 Revised:1997-09-16 Online:1998-03-15 Published:1998-03-15

摘要: 灵芝1~100 μg/mLL929细胞的生长无直接影响。在加灵芝与不加灵芝的条件下观察重组肿瘤坏死因子(rTNF)L929细胞的杀伤作用证明灵芝不影响rTNF的杀伤肿瘤活性。但灵芝0.01~10 μg/mL在试管内促进小鼠腹腔巨噬细胞产生TNF。巨噬细胞在试管内经灵芝预培养8 h,灵芝仍可增加巨噬细胞在LPS诱导下TNF的产生。此外,整体研究证明口服灵芝100~300 mg/kg qd×14可以增高由BCG启动及内毒素诱导的小鼠血清TNF水平。

关键词: 灵芝, 肿瘤坏死因子, 巨噬细胞

Abstract: Effects of Ganoderma lucidum (GL) on the cytotoxic activity of tumor necrosis factor (TNF) or its production were examined using murine fibroblast cell line (L929 cells). It was found that GL 1~100 μg/mL had no direct influence on the growth of L929 cells, and GL showed less synergistic activity on the cytotoxicity of rTNF. However, GL 0.01~10 μg/mL promoted TNF production by macrophages in vitro. Preincubated with GL for 8 h before LPS, macrophages produced higher TNF activity in vitro. Besides, GL 100~300 mg/kg po qd×14 enhanced the level of TNF in the serum of mice primed by BCG and induced by endotoxin.

Key words: Ganoderma lucidum, Tumor necrosis factor, Macrophage

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