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人参皂甙Rg1对老年人淋巴细胞蛋白质酪氨酸激酶活性的调控作用

刘俊达, 王舒, 刘红涛   

  1. 1. 卫生部北京老年医学研究所, 100730;
    2. 北京医科大学免疫学教研室, 100083
  • 收稿日期:1995-10-24 修回日期:1996-01-16 出版日期:1996-03-15 发布日期:1996-03-15

Modulatory Effect of Rg1 on the Activity of ProteinTyrosine Kinase of Lymphocytes in the Elderly

Jun-Da Liu, Shu Wang, Hong-Tao Liu   

  1. 1. Department of Immunologr. Beijing insiitule of Geriatrics. Beijing, 100730;
    2. Department of Molecular Immunologr Beijing Medical University, Beijing 100083
  • Received:1995-10-24 Revised:1996-01-16 Online:1996-03-15 Published:1996-03-15

摘要: 选择7名健康老年人为对象, 研究了人参皂甙Rg1对其淋巴细胞表面抗原。受体及蛋白质酪氨酸激酶(PTK)活性的作用, 用间接免疫荧光法测定了CD25CD45RACD45R阳性细胞百分率。单独PHA(5 μg/ml)培养72小时分别为38.3%±173%46.0%±15.1%526%±14.4%; 而用PHA(5 μg/ml)Rg1(μg/ml)联合培养时则分别为58.0%±12.5%64.1%±12.4%74.1%±8.0%。两者相比分别皆有显著性(P<0.05), ELISA法测定了经PHAPHA+Rg1培养30分钟和72小时细胞浆PTK的吸光度值, PHA组为0.120±0.020, PHA±Rg1组为0.138±0.015, (P<0001)。结果证明, Rg1显著升高PTK活性。据此得出几点结论, 并讨论广Rg1PTK的作用机理。

关键词: 淋巴细胞, Rg1, 受体, PTK

Abstract: The effect of Rg1, a saponin extracted froin Panax ginseng, on the phenotype, receptor and the activity of protein tyrosine kinase (PTK) of lymphocytes isolated from 7 healthy oldpersons were studied. The CD25, CD45RA and CD45RO phenotypes of lymphocytes were 4eter-mined by indirect immunofluorescence technique. The percentage of CD25, CD45RA and CD45RO positive lymphocytes was 38.3%±17.3%, 46.0%±15.1%, and 52.6%±14.1% respectively after incu-bation with PHA (5 μg/ml) for 72 hours. However, there were 58.0%±12.5%, CD25, 64.1% ± 12.4%, CD45RA, and 74.0%±8.0%, CD45RO positive cells in the presence of Rg, ( 1μg/ml) along with PHA(5 μg/ml) over the sanie period of incubation. A significant increase was induced by Rgi (P<0.05).The activities of PTK in the cytoplasm and membrane of lymphocytes were measured by ELISAmcthod after incubation with PHA or PHA+Rg1. The absorbance value of PTK activity in cytoplasm after 72 h incubation was 0.120±0.020 in PHA group, but 0.138±0.015 in PHA+Rg1 group. In thelymphocyte membrane, it was 0.374± 0.060 in PHA group and 0.403 ± 0.008 in PHA+Rg1 group(P<0.001). These results showed that Rgi significantly arid simultaneously increased both the PT Kactivity and the expression of phenotype of lymphocytes.

Key words: Lymphocyte, Rg1, Receptor, Protein tyrosine kinase

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