硒化卡拉胶对肿瘤坏死因子生成的影响

硒化卡拉胶对肿瘤坏死因子生成的影响

张罗修, 蒋建明, 贾永锋

上海医科大学药学院药理教研室药理教研室, 上海 200032

收稿日期:1992-09-28 修回日期:1993-04-06 出版日期:1994-06-15 发布日期:1994-06-15

Effect of к-Selenocarrageenan on the Production of tumor Necrosis Factor (TNF)

Luo-Xin Zhang, jian-Ming Jiang, Yong-Feng Jia

Department of pharmacology School of pharmacy, Shanghai Medical University, Shanghai 200032

Received:1992-09-28 Revised:1993-04-06 Online:1994-06-15 Published:1994-06-15

摘要/Abstract

摘要： 研究了硒化卡拉胶对小鼠腹腔巨噬细胞产生肿瘤坏死因子的影响。研究结果表明在试管内硒化卡拉胶0.1~10 μg/ml促进脂多糖(IPS)诱导巨噬细胞生成肿瘤坏死因子(TNF)。硒化卡拉胶0.1~10 μg/ml与巨噬细胞预培养8 h, 亦能促进TNF生成。但是在同一条件下硒化卡拉胶本身对靶细胞并无直接杀伤作用。培养系统中所含药物亦不影响靶细胞对重组TNP的反应性。整体研究表明硒化卡拉胶口服4, 12, 及36 mg/kg qd×15能促进经卡介苗及LPS诱导的TNF生成。

关键词: к-硒化卡拉胶, 肿瘤坏死因子

Abstract: The present study was designed to investigate the effect ofк-selenocarrageenan (Se-carra) on the production of tumor necrosis factor (TNF) from macrophages of micein vitro. It wasfound that Se-carra 0.1~10 μg/ml promoted the production of TNF from peritoneal macrophages of mice induced by LPS in vitro. It was demonstrated that 0.1~10μg/ml had no direct cytotoxic action onthe target cells. Besides, under the same condition Se-carra had no effect on the reaction of L929 cellsto r-TNF. Further experiment showed that peritoneal macrophages pretreated with Se-carra 0.1~10μg/ml for 8 h before LPS also enhanced the release of TNF.

Key words: к-Selenocarrageenan, Tumor necrosis factor (TNF)

Supporting:

张罗修, 蒋建明, 贾永锋. 硒化卡拉胶对肿瘤坏死因子生成的影响[J]. .

Luo-Xin Zhang, jian-Ming Jiang, Yong-Feng Jia. Effect of к-Selenocarrageenan on the Production of tumor Necrosis Factor (TNF)[J]. .

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