卵白素-脂质体复合物作为开管毛细管电色谱的手性选择剂分离盐酸美西律

doi:10.5246/jcps.2017.12.097

摘要/Abstract

摘要：

本研究开发了一种新的开管毛细管电色谱方法用于手性分离盐酸美西律, 其中使用卵白素-脂质体复合物作为固定相。卵白素被组装在包含DPPE和PS (80:20, mg%)的脂质体上, 两者复合体涂布在毛细管内壁。脂质体的表征用来评价涂层的均一性, 而D,L-色氨酸的分离度被用来验证涂层的分离效能。对于盐酸美西律的手性分离, 我们研究了影响分离效能的四个参数: 缓冲液的种类、pH、浓度和施加电压, 在最优的条件下, 盐酸美西律对映异构体得到较好的分离度。实验结果表明使用卵白素-脂质体复合物作为开管毛细管电色谱的固定相是可行的, 在药物手性分离上有着潜在的优势。

关键词: 卵白素, OTCEC, 手性分离, 脂质体, 盐酸美西律

Abstract:

In the present study, we developed a novel open-tubular capillary electrochromatographic method using avidin-phospholipidvesicle complex as the stationary phase for chiral separation of mexiletine hydrochloride. The avidin immobilized on the phospholipid vesicle consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) and L-α-phosphatidyl-L-serine (PS) (80:20, mg%) was coated in the capillary. The homogeneity and separation performance of the coating were evaluated in terms of phospholipid vesicle characterization and the resolution of D,L-Tryptophan. As for mexiletine hydrochloride, four vital parameters affecting the separation efficiency of coating capillary, including buffer type, buffer pH, buffer concentration and the applied voltage, were studied in detail. Under the optimum conditions, the enantiomers could be separated well with good resolution. All the satisfactory results indicated that this method using avidin-phospholipid vesicle complex as the stationary phase was suitable and feasible, which had great potential in pharmaceutical separation of enantiomers.

Key words: Avidin, OTCEC, Chiral separation, Phospholipid vesicle, Mexiletine hydrochloride

中图分类号:

R917

Supporting:

高也, 邱百灵, 梁启慧, 吴迪, 宋宇, 韩南银. 卵白素-脂质体复合物作为开管毛细管电色谱的手性选择剂分离盐酸美西律[J]. 中国药学(英文版), 2017, 26(12): 872-880.

Ye Gao, Bailing Qiu, Qihui Liang, Di Wu, Yu Song, Nanyin Han. Application of avidin-phospholipid vesicle complex as the stationary phase for chiral separation in open-tubular capillary electrochromatography[J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(12): 872-880.

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