La/Gd对高磷条件下SD大鼠骨髓基质干细胞分化的不同效应

doi:10.5246/jcps.2016.07.059

中国药学(英文版) ›› 2016, Vol. 25 ›› Issue (7): 535-545.DOI: 10.5246/jcps.2016.07.059

La/Gd对高磷条件下SD大鼠骨髓基质干细胞分化的不同效应

于彩霞, 龚娇, 黄健^*, 张天蓝, 王夔

北京大学医学部药学院化学生物学系, 北京 100191

收稿日期:2016-03-28 修回日期:2016-04-25 出版日期:2016-07-19 发布日期:2016-05-17
通讯作者: Tel.: +86-010-62015584, +86-010-82801539, E-mail: jhuang@bjmu.edu.cn, yucaixia0105@163.com

Comparison of effects on bone mesenchymal stem cells differentiation between lanthanum and gadolinium under high phosphate condition

Caixia Yu, Jiao Gong, Jian Huang^*, Tianlan Zhang, Kui Wang

Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2016-03-28 Revised:2016-04-25 Online:2016-07-19 Published:2016-05-17
Contact: Tel.: +86-010-62015584, +86-010-82801539, E-mail: jhuang@bjmu.edu.cn, yucaixia0105@163.com

摘要/Abstract

摘要：

镧系元素(Lns)中, 镧(La)和钆(Gd)在临床诊断和治疗中发挥着重要作用, 二者在临床中的应用会导致La和Gd 蓄积于骨骼内。La和Gd 物理化学性质相似, 当二者应用于肾功能不全患者时, 相似的性质是否会对骨代谢造成相似的生物学影响, 正在引起研究人员关注。本文比较了在高磷条件下LaCl₃和GdCl₃对大鼠骨髓间充质干细胞(BMSCs)分化的作用, 并探讨其可能机理。结果表明, 在高磷条件下, LaCl₃高浓度抑制BMSCs分化, 低浓度能够促进分化相关基因表达; 而GdCl₃促进细胞分化, 进一步研究发现, 培养过程中生成的含Lns的钙磷酸盐沉淀参与了在本实验中观察到的La和Gd对细胞分化的不同作用。La和Gd在原子序数上存在微小差异, 致使Gd对沉淀生成的促进作用更强; 含Gd的钙磷酸盐颗粒吸附蛋白量少于含La的钙磷酸盐颗粒, 导致含Gd的颗粒粒径较大。总之, Gd与La参与的颗粒理化性质上的不同部分导致了本实验中LaCl₃和GdCl₃对细胞分化作用的不同。

关键词: 镧, 钆, 高磷条件, 细胞分化

Abstract:

Lanthanum (La) and gadolinium (Gd) have been used in clinical diagnosis and treatment. The application of La and Gd in medication may cause accumulation of the elements in bone. Whether the similar physicochemical properties of these two Lanthanides (Lns) will lead to similar biological effects on bone metabolism in patients with severe kidney failureare key concerns of researchers.This study compared the inﬂuences of LaCl₃ and GdCl₃ on in vitro differentiation of bone mesenchymal stem cells (BMSCs) in high phosphate (Pi) medium. The results showed that LaCl₃ inhibited high Pi-induced BMSC differentiation at higher concentration and stimulated BMSC differentiation-related genes at lower concentration, while GdCl₃ promoted high Pi-induced BMSC differentiation. The emergence of insoluble Ln-containing calcium phosphate (CaP) was implicated in the process of LaCl₃ or GdCl₃ mediated osteogenic differentiation.The small differences in atomic number of La and Gd causedthe more promotive effect of GdCl₃on the precipitation of CaP compared with LaCl₃.Moreover, the content of serum proteins coprecipitated in Gd-containing particles was decreased compared with La-containing particles, leading to larger particle size of Gd-containing particles.The study demonstrated that those distinct physicochemical propertiesof Gd-containing particlesvs. La-containing particlesplayed an important role in modulatingthe different effects of GdCl₃and LaCl₃on BMSC differentiation.

Key words: Lanthanum, Gadolinium, High phosphate, Differentiation

中图分类号:

R916.3

Supporting:

于彩霞, 龚娇, 黄健, 张天蓝, 王夔. La/Gd对高磷条件下SD大鼠骨髓基质干细胞分化的不同效应[J]. 中国药学(英文版), 2016, 25(7): 535-545.

Caixia Yu, Jiao Gong, Jian Huang, Tianlan Zhang, Kui Wang. Comparison of effects on bone mesenchymal stem cells differentiation between lanthanum and gadolinium under high phosphate condition[J]. Journal of Chinese Pharmaceutical Sciences, 2016, 25(7): 535-545.

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La/Gd对高磷条件下SD大鼠骨髓基质干细胞分化的不同效应

Comparison of effects on bone mesenchymal stem cells differentiation between lanthanum and gadolinium under high phosphate condition

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