芍药内酯苷通过TLR4通路对大鼠溃疡性结肠炎的治疗作用及Tollip表达的调控作用

doi:10.5246/jcps.2016.05.041

中国药学(英文版) ›› 2016, Vol. 25 ›› Issue (5): 366-372.DOI: 10.5246/jcps.2016.05.041

芍药内酯苷通过TLR4通路对大鼠溃疡性结肠炎的治疗作用及Tollip表达的调控作用

方新华¹, 吴鑫², 朱雪梅³, 蔡皓⁴, 曹岗^2*

1. 杭州市中医院中药房, 浙江杭州 310007
2. 浙江中医药大学中药炮制技术研究中心, 浙江杭州 311401
3. 浙江中医药大学附属温州市中医院, 浙江温州 325000
4. 南京中医药大学药学院, 江苏南京 210023

收稿日期:2016-01-24 修回日期:2016-02-15 出版日期:2016-05-30 发布日期:2016-03-12
通讯作者: Tel.: +86-571-87195895, E-mail: caogang33@163.com
基金资助:
National Natural Science Foundation of China (Grant No. 81573602), the Project of Science and Technology for Chinese Medicine of Zhejiang Province, China (Grant No. 2013KYB183), the Chinese Medicine Research Program of Zhejiang Province, China (Grant No. 2014ZQ008, 2016ZA183, 2015ZQ011), and Zhejiang Province Association of Traditional Chinese Medcine (Grant No. 2013103).

Albiflorin attenuates inflammatory injury by regulating the TLR4 signaling pathway and its negative regulating factor Tollip in experimental models of ulcerative colitis

Xinhua Fang¹, Xin Wu², Xuemei Zhu³, Hao Cai⁴, Gang Cao^2*

1. TCM Dispensary, Traditional Chinese Medical Hospital of Hangzhou, Hangzhou 310007, China
2. Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 311401, China
3. Department of Pharmacy, Wenzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, Wenzhou, 325000 China
4. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

Received:2016-01-24 Revised:2016-02-15 Online:2016-05-30 Published:2016-03-12
Contact: Tel.: +86-571-87195895, E-mail: caogang33@163.com
Supported by:
National Natural Science Foundation of China (Grant No. 81573602), the Project of Science and Technology for Chinese Medicine of Zhejiang Province, China (Grant No. 2013KYB183), the Chinese Medicine Research Program of Zhejiang Province, China (Grant No. 2014ZQ008, 2016ZA183, 2015ZQ011), and Zhejiang Province Association of Traditional Chinese Medcine (Grant No. 2013103).

摘要/Abstract

摘要：

芍药内酯苷为白芍中的主要活性成分。本文研究芍药内酯苷通过调节TLR4通路和Tollip表达治疗实验性溃疡性结肠炎的药效作用。应用三硝基苯磺酸治疗大鼠溃疡性结肠炎模型,通过测定TNF-α、IL-1、IL-10、5-HT、MPO, 结合病理组织切片和TLR4通路和Tollip相关蛋白的表达,观察芍药内酯苷对溃疡性结肠炎的疗效。研究结果显示,芍药内酯苷可通过显著降低TNF-α、IL-1、IL-10、5-HT水平,显著降低TLR4、MyD88和NF-κB p65的蛋白表达和上调Tollip的表达,发挥治疗溃疡性结肠的作用。由此可见,芍药内酯苷可作为治疗溃疡性结肠炎的潜在天然药物。

关键词: 芍药内酯苷, 溃疡性结肠炎, TLR4信号通路, Tollip

Abstract:

Albiflorin (AF) is the main active component extracted from Paeoniae Radix Alba. This study investigated the efficacyof AF in attenuating inflammatory injury by regulating the TLR4 signaling pathway and its negative regulating factor Tollip in an experimental ulcerative colitis (UC) model. We administrated trinitrobenzene sulfonic acid for 21 d to induce UC in rats. The efficacy of AF in attenuating UC was assessed using various biochemical markers, such as tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), interleukin-10 (IL-10), 5-hydroxytryptamine (5-HT), and tissue myeloperoxidase (MPO), along with histopathological studies on toll-like receptor-4 (TLR-4) signaling pathway and its negative regulating factor Tollip. The results showed that AF can significantly downregulate the levels of TNF-α, IL-1, IL-10, and 5-HT. AF decreased the activation of TLR4, MyD88, and NF-κB p65 protein expression by increasing Tollip expression. AF can relieve symptoms of UC by suppressing the activation of the TLR4 signaling pathway and upregulating its negative regulating factor Tollip. Therefore, AF may be a potential natural product for treating UC.

Key words: Albiflorin, Ulcerative colitis, TLR4 signaling pathway, Tollip

中图分类号:

R962.1

Supporting:

方新华, 吴鑫, 朱雪梅, 蔡皓, 曹岗. 芍药内酯苷通过TLR4通路对大鼠溃疡性结肠炎的治疗作用及Tollip表达的调控作用[J]. 中国药学(英文版), 2016, 25(5): 366-372.

Xinhua Fang, Xin Wu, Xuemei Zhu, Hao Cai, Gang Cao. Albiflorin attenuates inflammatory injury by regulating the TLR4 signaling pathway and its negative regulating factor Tollip in experimental models of ulcerative colitis[J]. Journal of Chinese Pharmaceutical Sciences, 2016, 25(5): 366-372.

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[2]	赵敏敏, 高萌, 田玉路, 杜英峰, 王春英, 许慧君, 张兰桐, 王巧. HPLC-MS/MS法研究白芍总苷在大鼠体内的药代动力学和组织分布[J]. 中国药学(英文版), 2014, 23(6): 403-411.
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芍药内酯苷通过TLR4通路对大鼠溃疡性结肠炎的治疗作用及Tollip表达的调控作用

Albiflorin attenuates inflammatory injury by regulating the TLR4 signaling pathway and its negative regulating factor Tollip in experimental models of ulcerative colitis

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