HPLC-MS/MS法研究白芍总苷在大鼠体内的药代动力学和组织分布

doi:10.5246/jcps.2014.06.054

中国药学(英文版) ›› 2014, Vol. 23 ›› Issue (6): 403-411.DOI: 10.5246/jcps.2014.06.054

HPLC-MS/MS法研究白芍总苷在大鼠体内的药代动力学和组织分布

赵敏敏, 高萌, 田玉路, 杜英峰, 王春英, 许慧君, 张兰桐, 王巧^*

河北医科大学药学院药物分析教研室, 河北石家庄 050017

收稿日期:2014-04-23 修回日期:2014-05-04 出版日期:2014-06-28 发布日期:2014-05-05
通讯作者: Tel.: 86-311-86266419, Fax: 86-311-86052053
基金资助:
Natural Science Foundation of Hebei Province of China (Grant No. C2011206158, 08B031) and Hundreds of Innovative Talents Project of Hebei Education Department of China.

Pharmacokinetic and tissue distribution studies of paeoniflorin and albiflorin in rats after oral administration of total glycosides of paeony by HPLC-MS/MS

Minmin Zhao, Meng Gao, Yulu Tian, Yingfeng Du, Chunying Wang, Huijun Xu, Lantong Zhang, Qiao Wang^*

Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China

Received:2014-04-23 Revised:2014-05-04 Online:2014-06-28 Published:2014-05-05
Contact: Tel.: 86-311-86266419, Fax: 86-311-86052053
Supported by:
Natural Science Foundation of Hebei Province of China (Grant No. C2011206158, 08B031) and Hundreds of Innovative Talents Project of Hebei Education Department of China.

摘要/Abstract

摘要： 建立HPLC-MS/MS法测定大鼠血浆和组织样品中芍药苷和芍药内酯苷, 研究白芍总苷在大鼠体内的药代动力学和组织分布特征。采用固相萃取法进行样品处理, 色谱柱为C₁₈柱, 流动相为乙腈-0.05%甲酸溶液 (20:80, v/v), ESI源, 负离子多反应监测模式。方法学验证结果均符合要求。白芍总苷灌胃给与大鼠后, 主要成分芍药苷和芍药内酯苷迅速吸收并达到峰值, 二者具有相似的药动学特征, 在组织中分布广泛, 并可透过血脑屏障。

关键词: 白芍总苷, 固相萃取, 芍药苷, 芍药内酯苷, 液质联用

Abstract:

A simple, sensitive and specific high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method combined with solid phase extraction has been developed and validated for the simultaneous quantification of paeoniflorin and albiflorin in rats plasma and tissue homogenate after administration of total glycosides of paeony (TGP). Chromatographic separation was achieved on a C₁₈column (150 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile–0.05% formic acid aqueous (20: 80, v/v) at a flow rate of 0.5 mL/min. The analytes were detected with triple-quadrupole mass spectrometer using turbo ion spray with negative ionization in the multiple reaction-monitoring (MRM) modes. The results of method validationconformed to the requirements for the determination of biological samples. This method was successfully applied to the pharmacokinetics and tissue distribution study of TGP in rats. The results showed that paeoniflorin and albiflorin were absorbed and reached peak value quickly, and had the similar pharmacokinetic characteristics. Both of them underwent a rapid and wide distribution in the tissues throughout the whole body, among which stomach was the main distribution tissue. Moreover, they had the ability to cross the blood-brain barrier.

Key words: Total glycosides of paeony, Solid-phase extraction, Albiflorin, Paeoniflorin, HPLC-MS/MS

中图分类号:

R969

Supporting: Natural Science Foundation of Hebei Province of China (Grant No. C2011206158, 08B031) and Hundreds of Innovative Talents Project of Hebei Education Department of China.

赵敏敏, 高萌, 田玉路, 杜英峰, 王春英, 许慧君, 张兰桐, 王巧. HPLC-MS/MS法研究白芍总苷在大鼠体内的药代动力学和组织分布[J]. 中国药学(英文版), 2014, 23(6): 403-411.

Minmin Zhao, Meng Gao, Yulu Tian, Yingfeng Du, Chunying Wang, Huijun Xu, Lantong Zhang, Qiao Wang. Pharmacokinetic and tissue distribution studies of paeoniflorin and albiflorin in rats after oral administration of total glycosides of paeony by HPLC-MS/MS[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(6): 403-411.

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HPLC-MS/MS法研究白芍总苷在大鼠体内的药代动力学和组织分布

Pharmacokinetic and tissue distribution studies of paeoniflorin and albiflorin in rats after oral administration of total glycosides of paeony by HPLC-MS/MS

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