Research on accurate quantitation technique using liquid chromatography coupled with triple quadrupole mass spectrometry and experiment optimization—take a novel diuretic as an example

doi:10.5246/jcps.2022.03.016

Abstract

Abstract:

Liquid chromatography coupled with the triple quadrupole mass spectrometry (LC-QQQ MS) technique is the most commonly used technique for quantitative analysis. It is widely used in pharmacology, targeted metabolomics, Chinese medicine quality control, and other research fields. The technique monitors only characteristic precursors and product ions through multiple reaction monitoring (MRM) mode and can detect targeted molecules in complex matrix with high specificity and sensitivity. In the present study, a diarylamide derivative diuretic was used as an example to introduce the method establishment and parameter optimization of this liquid chromatography-mass spectrometry technique. Diuretic and its internal standard could be completely separated within a 5-min gradient, and the quantitative linear range was 1–1000 ng/mL with R²= 0.9996 in practical samples. This study showed that the key to the method development for LC-QQQ MS was the selection of the LC mobile phase, the elution gradient, the declustering potential, and the collision energy of compounds in MS.

Key words: Liquid chromatography, Triple quadrupole mass spectrometry, Method establishment, Diuretic

Supporting:

Wen Ma, Yue Xu, Shuyuan Wang, Jun Li, Baoxue Yang. Research on accurate quantitation technique using liquid chromatography coupled with triple quadrupole mass spectrometry and experiment optimization—take a novel diuretic as an example[J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(3): 184-191.

Figures/Tables 7

Table 1. MS ion source parameter optimization.

Table 2. RPLC-MS gradient for separation.

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