Development and validation of an LC-MS/MS method for quantification of dipalmitoylphosphatidylcholine as a promising biomarker for renal failure in urine

doi:10.5246/jcps.2015.02.008

Abstract

Abstract:

Urinary phospholipids are shown to be sensitive biomarkers for kidney injury. Many works have been reported on qualitative analysis of phospholipids and relative components in human urine while quantitative analysis is rare. We have therefore developed a reliable and convenient quantitative method to evaluate the accuracy and specificity of dipalmitoylphosphatidylcholine (DPPC) as a biomarker for kidney injury diagnosis. Chromatographic separation was achieved using a HILIC Silica Column (2.1 mm×50 mm, 5 µm).Gradient elution was performed with 5 mM ammonium formate (0.1% formic acid) and acetonitrile (0.1% formic acid), at a flow rate of 0.3 mL/min. This method was validated in a linear range of 2–200 ng/mL DPPC with inter-day precision of less than 6.5% and accuracy within 111.2% in human urine. Recovery rate, stability, dilution effect and matrix effect also met the requirements for drug evaluation and research issued by FDA. As the first HPLC-MS/MS method for quantitative determination of DPPC, it has been successfully applied to determine levels of DPPC in clinical urine samples and evaluated for potential use in the measurement of DPPC as a biomarker for kidney injury.

Key words: Dipalmitoylphosphatidylcholin, Kidney injury, Liquid chromatography-tandem mass spectrometry, Urin

CLC Number:

Supporting:

Dandan Li, Xin Xiong, Qiong Bai, Wenling Yang, Rongsheng Zhao, Aihua Zhang. Development and validation of an LC-MS/MS method for quantification of dipalmitoylphosphatidylcholine as a promising biomarker for renal failure in urine[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(2): 73-79.

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